用于检测细胞表面蛋白的抗体包括:购自ebioscience的CD3e、CD4、CD8a、CD19、NK1.1、F4/80、CD45、CD11b、CD45.1、CD45.2、Ki67、Ly6C和购自biolegend的CD115、Ly6G、和Siglec-F。IL4Rα抗体购自R&D。 通过在消化前以和胶原酶为1:1000比例加入GolgiPlug,
骨髓来源的抑制性细胞(Myeloid-derived suppres洋战尼行sor cells,MDSCs)是骨髓来源的一群异质性细胞,是树突状细胞(dendritic cells,DCs)、巨噬细胞和(或)粒细胞的前体,具有显著抑制免富烈绍学治盾济条免疫细胞应答的能力。 小鼠MDSCs的表型为CD11b+Gr-1+,分两个亚型:粒细胞样的MDSCs(CD11b+Ly6C-Ly6G+)和...
为进一步明确具体哪一类免疫细胞参与此过程,作者应用流式细胞技术分析了Ang II灌注的动脉中炎症细胞,结果表明CD45+CD11b+Ly6C+Ly6G-F4/80+巨噬细胞呈现时间依赖性地增加。这些结果提示,骨髓巨噬细胞中CD11b的表达上调可能参与高血压的发生发...
The results show that the depletion of peritoneal macrophages (CD11b+F4/80+) and blood monocyte (CD11b+Ly6G-Ly6C-) could be achieved after two injections of DT, but the neutrophil (CD11b+Gr-1+) were increased transiently. Results also found that the depletion of CD11b+ myeloid cells ...
另一方面,糖代谢限制可以直接诱导Ly6GlowG-MDSC的凋亡,而不是Ly6Ghigh亚群的凋亡。总之,这些结果表明,禁食治疗诱导的糖代谢限制减弱了免疫抑制环境,增强了CD3+T细胞的激活,为加强基于免疫的癌症干预提供了潜在的解决方案。 Intermittent fast...
RESEARCH ARTICLE Open AccessCD11b + Ly6C ++ Ly6G - cells show distinct functionin mice with chronic inflammation or tumorburdenEva Källberg, Martin Stenström, David Liberg, Fredrik Ivars and Tomas Leanderson *AbstractBackground: S100A9 has been shown to be important for the function of so ...
The CD11b+ Ly6Chigh monocytes have been shown to play a role during the onset of inflammatory process in the Listeria-loaded brain.38 More recently, CD31pos:Ly-6Cpos:CD11bpos:LY-6Glow leukocytes present in the bone marrow as early as 24 hours after the intravenous inoculation of Listeria ...
To this end, we sorted CD11b+ cells using FACS with regard to the expression of the Ly6G and Ly6C markers into three populations; Ly6C+G+, Ly6C+G- and Ly6C++G-, as shown in Figure 1A. RNA was prepared from the isolated cell populations and S100A9 expression was measured using Q-...
However, sorted CD11b+Gr1+ cells (comprising more than 90% of CD11b+Ly6G+Ly6Cint cells) from MPA-treated tumor bearing mice displayed a more pronounced suppressive activity on NK cell degranulation in response to YAC-1 cells and a stronger inhibition of IFN-g production of NK cells in ...
Liver MDSCs in TGF-β1 knockout mice are defined as CD11b+Ly6G−Ly6Chigh monocytic cells and their suppressor function is dependent on nitric oxide (NO) production by iNOS (Cripps et al., 2010). In contrast, liver MDSCs in HBV-Tg mice are characterized as CD11b+Gr-1+ (Chen et al...