黑框圈出的细胞群为Ly6GhighG-MDSC,红框圈出的细胞群为Ly6GlowG-MDSC(图4A1和B1)。4T1和4T07对照组Ly6GlowG-MDSC亚群的百分比分别为(13.8±2.0)%和(14.2±2.7)%,而禁食组的比例分别降至(6.2±4.7)%和(4.5±3.8)%(图4A2...
请问组织中LY6C+CD11b+LY6G-是什么细胞? 展开 网友 1 最佳答案 回答者:网友 骨髓来源的抑制性细胞(Myeloid-derived suppres洋战尼行sor cells,MDSCs)是骨髓来源的一群异质性细胞,是树突状细胞(dendritic cells,DCs)、巨噬细胞和(或)粒细胞的前体,具有显著抑制免富烈绍学治盾济条免疫细胞应答的能力。 小鼠MDSC...
为进一步明确具体哪一类免疫细胞参与此过程,作者应用流式细胞技术分析了Ang II灌注的动脉中炎症细胞,结果表明CD45+CD11b+Ly6C+Ly6G-F4/80+巨噬细胞呈现时间依赖性地增加。这些结果提示,骨髓巨噬细胞中CD11b的表达上调可能参与高血压的发生发...
图11A显示了流式细胞术分析结果,图中CD45+F4/80hiSiglec-F-定义为脂肪组织巨噬细胞,CD45+F4/80+Siglec-F+为嗜酸性粒细胞,CD45+NK1.1+为自然杀伤细胞,CD45+Ly6G+为中性粒细胞。 图11B显示了脂肪组织的淋巴细胞流式细胞术分析结果,图中包括CD19+B细胞、CD3+CD8+T细胞、和CD3+CD4+T细胞。 图11C显示了流式...
M., Pencovich, N. CD11b + Ly6G + myeloid-derived suppressor cells promote liver regeneration in a murine model of major hepatectomy.doi:10.1096/fj.201801733RJohn Wiley & Sons, LtdThe FASEB Journal
Ly6g-phycoerythrin was then used to negatively gate out all neutrophils, and all lymphocytes were gated out based on their lower FSC-A and SSC-A profile. This allowed FACS analysis on the remaining M^/monocyte subpopulations. AMFs were discriminated from dendritic cells based on their high auto...
To this end, we sorted CD11b+ cells using FACS with regard to the expression of the Ly6G and Ly6C markers into three populations; Ly6C+G+, Ly6C+G- and Ly6C++G-, as shown in Figure 1A. RNA was prepared from the isolated cell populations and S100A9 expression was measured using Q-...
BM monocytes (Ly6ChiLy6Gint) in E. coli-infected vagotomized and Chrna7−/−Itgam−/− mice were increased compared to E.coli-infected wildtype mice, whereas BM neutrophils (Ly6CintLy6Ghi) were not different among these three groups (Supplementary Figure S11C–E). These findings ...
Cells were stained with the following mAbs (BD Pharmingen): anti-CD11c (HL3, biotinylated or phycoerythrin conjugated), anti-CD11b (M1/70, allophycocyanin conjugated), or/and anti-Ly6G/C (Gr-1) (RB6-8C5, phycoerythrin or fluorescein isothiocyanate (FITC) conjugated). Allophycocyanin or peridin...
RESEARCH ARTICLE Open AccessCD11b + Ly6C ++ Ly6G - cells show distinct functionin mice with chronic inflammation or tumorburdenEva Källberg, Martin Stenström, David Liberg, Fredrik Ivars and Tomas Leanderson *AbstractBackground: S100A9 has been shown to be important for the function of so ...