答:Gr-1单抗通常认为是粒细胞标记,但实际上,它既可结合Ly-6G也可结合Ly-6C。大多数 Gr1+ CD11b- 细胞是 Ly6C+ LyG-,Ly6C可表达在浆细胞、部分粒细胞和T 细胞(包括记忆 T 细胞)表达。所以Gr1+ CD11b-细胞可能CD8是阳性的,如果补做CD8是阳性的...
2000. Nitric oxide- producing CD11b+Ly-6G (Gr-1)+CD31(ER-MP12)+ cells in the spleen of cyclophosphamide-treated mice: implications for T-cell responses in immunosuppressed mice. Blood 95:212.I Angulo,F Gomez de las Heras,JF Garcia-Bustos,D Gargallo,MA Munoz-Fernandez,M Fresno.Nitric ...
上图:用小鼠CD11b+细胞分选试剂盒分离小鼠脾细胞中的CD11b+细胞。利用Anti-Mouse Ly-6G(Gr-1)eFluor®450(货号48-5931)和Anti-Mouse CD 11b PE(货号12-0112)标记细胞,对分选前(左)或分选后(右)的细胞进行流式分析。 下图:用小鼠CD11b+细胞试剂盒分离小鼠骨髓细胞中的CD11b+细胞。利用Anti-Mouse Ly-6G...
but their high display levels of Ly-6C prompted us to test whether these HDE-induced APCs were indeed bona fide cDCs, or were instead derived from monocytes. We purified multiple APC subsets and from the lung, prepared RNA from them, and performed bulk RNA-sequencing (RNA-Seq). ...
Rat monoclonal IgG2a antibody against mouse Ly-6G Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal Goat polyclonal antibody against mouse IgG (H+L) Quality Statement: PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGN...
PCR analysis revealed hardly detectable expression of Ido1 mRNA at steady state in all three mice groups, whereas a sharp increase could be observed at early stages of inflammation in WT control and in Clec4a4-DTR mice (Figure 6g). Interestingly and consistent with the inflammation-prone ...
1x10^6 mouse bone marrow cells were surface stained with 0.2 ug PE Anti-Mouse CD11b (M1/70) (PE-65055, Clone:M1/70) or 0.2 ug PE Rat IgG2b Isotype Control (LTF-2) (PE-65211, Clone: LTF-2), and 0.2 ug CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (CL...
Alexa Fluor® 594 anti-mouse/human CD11b Antibody - CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40.
5,6 These iMacs expressed the markers CD11b (Mac-1) and Gr-1 (Ly-6G), and removal of either CD11b+ or Gr-1+ splenocytes restored the deficient T-lymphocyte responses.5,6 Figure1A shows the morphology of cells selected for the presence or absence of CD11b and Gr-1 from spleens...
Isolate highly purified mouse MDSCs (CD11b+Gr1+) from splenocytes, bone marrow, or peripheral blood in as little as 18 minutes with EasySep™.