迁徙基质金属蛋白酶-2目的:探讨组织蛋白酶D(cathepsin D)对CD11b+髓系来源细胞(BMDCs)体外增殖,迁徙和侵袭的影响,并初步探索侵袭机制.方法:建立预转移期内胰腺癌原位瘤裸鼠模型L3.6pl组或Colo357组和无瘤荷裸鼠模型,流式细胞术检测骨髓和肝脏中CD11b+髓系来源细胞的比例,MTT检测细胞增殖能力,Tran-swell侵袭小室...
The role of CD11b+ BMDCs in the angiogenesis and vasculogenesis processes of Lewis tumors one week after 20 Gy local irradiation. Double immunostaining of CD11b with CD31, a-SMA, MMP-9 and iNOS for tumor sections were used to demonstrate the role of CD11b+ BMDCs in tumor neovasculature....
Furthermore, SBAs are primarily taken up by clathrin-mediated endocytosis and uptake induces lipid bodies and antigen translocation to the cytosol in MHCIIloCD11bhi BMDCs and BMDMs. Importantly, BMDMs treated with SBAs exhibit cross-presentation leading to potent CD8 T cells activation. Our findings...