6. Phenol red and serum will not interfere with the detection of CCK8 method and can be elimin...
向各孔中加入10μl的CCK-8溶液。如果待测溶液有还原性，测定不含细胞，但含有CCK-8的待测溶液在450...
结论ConA和LPS刺激的Balb/C小鼠脾细胞增殖实验的最佳条件为,轻压制备脾细胞,脾细胞浓度为5×106/mL,制备当天直接种板,ConA的刺激浓度为2~10 μg/mL,LPS的刺激浓度为10~50 μg/mL。 Study on determination conditions for lymphocytic proliferation by CCK-8 method in mice WANG yuan-yuan1 ,TAN xiao-yuan...
Inthisarticle,wecomparedtheapplicationofMTTandCCK8methodsindetectingthecytotoxiceffectsofantiviralactiveingredientsoftraditionalChinesemedicine.Experimentalcomparisonshowedthatthetwomethodshavesimilareffectsindetectingcelltoxicity,butCCK8methodiseasiertooperateandsuitableforhigh-throughputdrugscreening.Keywords:MTTmethod,CCK8...
结论CCK-8法在孵育4 h后及酶标仪450 nm波长下检测兔筋膜成纤维细胞的灵敏度最高。 标签:CCK-8法;成纤维细胞;兔;增殖活性;测试条件 An experimental research on the best test conditions of CCK-8 method detecting rabbit fascia fibroblasts(RFFBs) proliferation activity XIN Pei-cheng,YANG Sheng,LU Jian-...
Cell Counting Kit-8,简称CCK-8试剂盒,是一种基于WST-8而广泛应用于细胞增殖和细胞毒性的快速、高灵敏度检测的试剂盒。 WST-8是一种类似于MTT的化合物,在电子耦合试剂存在的情况下,可以被线粒体内的一些脱氢酶还原生成橙黄色的 formazan(参考图1)。细胞增殖越多越快,则颜色越深;细胞毒性越大,则颜色越浅。对...
(P<0.05).Calcein-AM/PIdouble-stainingflowdetectionmethodcanbetterdistinguishbetweenlivingcellsandapoptoticcells,andtheaccuracy,stabilityandrepeatabilityoftheresultsarestrongerthanCCK-8method.Conclusion:FlowcytometryisabetterandmoreaccuratemethodthanCCK-8inthedetectionofimmuno-cytotoxicactivity.【Keywords】breastcancer,...
A Simple and Sensitive Method for Quantifying Cell Proliferation and Cytotoxicity with the CCK-8 Assay [移除了无效网址] 总结 CCK8实验是一种简单、快速、灵敏的细胞活性检测方法,具有广泛的应用前景。在进行CCK8实验时,应注意细胞接种密度、培养条件、CCK-8试剂用量和吸光度值测量等因素,以获得准确可靠的实验...
本文主要比较了MTT法和CCK8法在检测中药抗病毒活性成分细胞毒性方面的应用。通过实验比较,发现两种方法在检测细胞毒性方面具有相似的效果,但CCK8法操作更为简便,适用于高通量药物筛选。 关键词:MTT法、CCK8法、中药抗病毒活性成分、细胞毒性 Abstract Inthisarticle,wecomparedtheapplicationofMTTandCCK8methodsindetecting...
Conclusion The optimized CCK-8 protocol is a sensitive,convenient and stable quantitative method to evaluate lymphocyte proliferation. This result can provide evidence in screening of immunomodulating drugs and investigation of their immunopharmacology. [Key words] CCK-8;PBMC;Lymphocyte proliferation;...