4. Add CCK-8: There are two methods, directly add 10ul of CCK-8 solution to each well, or...
Inthisarticle,wecomparedtheapplicationofMTTandCCK8methodsindetectingthecytotoxiceffectsofantiviralactiveingredientsoftraditionalChinesemedicine.Experimentalcomparisonshowedthatthetwomethodshavesimilareffectsindetectingcelltoxicity,butCCK8methodiseasiertooperateandsuitableforhigh-throughputdrugscreening.Keywords:MTTmethod,CCK8...
摘要关键词:MTT法、CCK8法、中药抗病毒活性成分、细胞毒性Inthisarticle,wecomparedtheapplicationofMTTandCCK8methodsindetectingthecytotoxiceffectsofantiviralactiveingredientsoftraditionalChinesemedicine.Experimentalcomparisonshowedthatthetwomethodshavesimilareffectsindetectingcelltoxi摘要city,butCCK8methodiseasiertooperateand...
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Inthisarticle,wecomparedtheapplicationofMTTandCCK8methodsindetectingthecytotoxiceffectsofantiviralactiveingredientsoftraditionalChinesemedicine.Experimentalcomparisonshowedthatthetwomethodshavesimilareffectsindetectingcelltoxicity,butCCK8methodiseasiertooperateandsuitableforhigh-throughputdrugscreening. Keywords:MTTmethod,CCK...
摘要 关键词：MTT法、CCK8法、中药抗病毒活性成分、细胞毒性Inthisarticle,wecomparedtheapplicationofMTTandCCK8methodsindetectingthecytotoxiceffectsofantiviralactiveingredientsoftraditionalChinesemedicine.Experimentalcomparisonshowedthatthetwomethodshavesimilareffectsindetectingcelltoxi 摘要 city,butCCK8methodiseasierto...
摘要关键词:MTT法、CCK8法、中药抗病毒活性成分、细胞毒性Inthisarticle,wecomparedtheapplicationofMTTandCCK8methodsindetectingthecytotoxiceffectsofantiviralactiveingredientsoftraditionalChinesemedicine.Experimentalcomparisonshowedthatthetwomethodshavesimilareffectsindetectingcelltoxi ...
1. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 1983, 65(1-2): 55-63. 2. Zhang C, et al. CCK-8 based OCT1 activity assay for selectively assessing the uptake clearance of metform...
(TCM).Inourstudy,MTTandCCK⁃8 wereusedtotestthecytotoxicityoftwo promisingantiviralcomponentsinaTCMnamedAndrographispaniculata,andrographolideand14⁃deoxy⁃11, 12⁃didehydroandrographolideinhumanlungepithelialA549cellline.Cellviabilitiesfromthetwomethods wereidenticalthatnon⁃toxicconcentrationsofandrographolide...
1. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 1983, 65(1-2): 55-63. 2. Zhang C, et al. CCK-8 based OCT1 activity assay for selectively assessing the uptake clearance of metform...