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Detection of fluorescence was performed as follows: GFP, excitation at 488 nm (Ar/Kr laser), scanning at 500 to 535 nm; yellow fluorescent protein (bimolecular fluorescence complementation), excitation at 514 nm (Ar/Kr laser), scanning at 525 to 600 nm; OFP, excitation at 543 nm (He/Ne...
YFP fluorescence was examined at 514 nm (excitation) using an argon laser with an emission band of 515–530 nm. 4.5. Measurement of Na+ and K+ Content For measurement of Na+ and K+ content in plant tissues, the seedlings of wild-type and transgenic Arabidopsis plants and COM lines were...