与贴壁细胞相比,源自乳腺癌细胞的肿瘤球表现出低得多的CAV-1水平。用siRNA沉默CAV-1可诱导干性,这可通过增加白细胞分化抗原44高表达/热稳定抗原低表达(CD44high/CD24low)细胞数量、乙醛脱氢酶(ALDH)活性、干性相关基因的表达和肿瘤球形成来证明。最近,CD44high/CD24low和ALDH+表型不常用于鉴定CSC,因为即使在相同分...
本发明公开一种Cav-1低表达的SMMC-7721KD细胞系的构建方法.具体采用Cav-1SiRNA干扰技术构建Cav-1稳定低表达的SMMC-7721KD细胞系.经过设计引物,扩增目的片段,构建Cav-1SiRNA重组质粒,进行质粒转染,然后对转染后的细胞进行博莱霉素筛选,传代,建立稳定低表达的SMMC-7721KD细胞系,最终获得阳性重组细胞系,能够沉默SMMC-...
CAV1 siRNA reduces membrane estrogen receptor-α levels and attenuates sexual receptivity. 喜欢 0 阅读量: 16 作者:Amy,Christensen,Paul,Micevych 摘要: Although classic estrogen receptors (ER) have been proposed to mediate estradiol signaling, it has been relatively recently that mechanisms of ...
addition, immunoblotting and immunofluorescence staining, subcellular fractionation, biotin labeling of cell surface proteins, and proximity ligation assay were employed to detect expression and localization of proteins in the retina or endothelial cells (ECs) upon knockdown of Cav-1 with Cav-1 siRNA. ...
CAV‑1 knockdown abrogates the inhibitory effect of Zed in HBSMCs To further verify whether CAV-1 expression is required for Zed's inhibitory effects on HBSMC proliferation, we next employed an siRNA knockdown strategy to reduce CAV-1 expression. First, we explored the best transfec- tion ...
6a, b). Then, primary neurons transfected with Cav-1 siRNA or control were treated with OGD and incubated with DiI-labeled EVs derived from HUVECs. We found that transfection of Cav-1 siRNA resulted in obvious reduction of DiI-EVs in neurons as determined by MFI (Fig. 6c). The level ...
5 Westernblot analysis of ovarian cancer cell lysates upon Cav1 silencing by siRNA treatment a. Cav1 expression was silenced under Cav1 siRNA treatment, but not under CTL siRNA treatment, in HeyA8 and SKOV3-ip1 cell lines. b. Silencing Cav1 using siRNA1 resulted in increased PDGFRβ ...
用脂质体法转染siRNA能明显抑制MCF-7细胞CAV1基因表达,RT-PCR扩增26个循环后未检测到CAV1 mRNA产物条带;转染组与未转染组相比,转染组CAV1蛋白水平下降67.6%(t=14.19,P<0.01);与RNA干扰对照组相比,差异有统计学意义(P<0.01,图4)。 图4 RNA干扰CAV1基因后的鉴定Fig.4 Effects of siRNA on the expression ...
链/]~RNA(siRNA)购于美 国Santa Cruz公司 ,转 染方法按试 剂说 明书进行 :MCF一7细胞 生长于 6孔板至60%～70%汇合 ,用无血清培养液静置 24 h,5 L siRNA与5 L RNA干扰转染 试剂 (Santa Cruz公司,美国)混合于l90 L无血清的 RPMI一1 640培养液 ,静置 30 min后滴加 到培养 ...
3.取N}玎LFs第3.7代使用,由上海吉玛制药技术有限公司设 计、制备得到3个不同序列的正常人caVl.siRNA,再将实验分为7 组,分别为空白对照组(N组)、TGF—p1(10ng/m1)处理组(M组)、 Co—siRNA转染组、Co.siIⅢA+TGF.p1(1 Ong/m1)处理组、 cavl一siRNA转染组、cavl-siRNA+TGF.p1(10ng/m1)处理组、 ...