用siRNA沉默CAV-1可诱导干性,这可通过增加白细胞分化抗原44高表达/热稳定抗原低表达(CD44high/CD24low)细胞数量、乙醛脱氢酶(ALDH)活性、干性相关基因的表达和肿瘤球形成来证明。最近,CD44high/CD24low和ALDH+表型不常用于鉴定CSC,因为即使在相同分子的乳腺癌亚型中,它们的表达也不一致。对它们的功能进行系统的比较...
CAV1 siRNA reduces membrane estrogen receptor-α levels and attenuates sexual receptivity. 喜欢 0 阅读量: 16 作者:Amy,Christensen,Paul,Micevych 摘要: Although classic estrogen receptors (ER) have been proposed to mediate estradiol signaling, it has been relatively recently that mechanisms of ...
本发明公开一种Cav-1低表达的SMMC-7721KD细胞系的构建方法.具体采用Cav-1SiRNA干扰技术构建Cav-1稳定低表达的SMMC-7721KD细胞系.经过设计引物,扩增目的片段,构建Cav-1SiRNA重组质粒,进行质粒转染,然后对转染后的细胞进行博莱霉素筛选,传代,建立稳定低表达的SMMC-7721KD细胞系,最终获得阳性重组细胞系,能够沉默SMMC-...
Con: control, Cav1 siRNA: Cav-1 depleted, T: total, S: soluble, M: membranous particular. *p < 0.05, **p < 0.01, ***p < 0.001 Full size image To confirm the findings, we performed immunofluorescence staining of VEGFR2 and VE-Cadherin in ECs and quantified their ...
CAV1 siRNA was used to validate our findings in vitro. In PDGF-stimulated HBSMCs, Zed significantly inhibited excessive proliferation of HBSMCs. Proteomic analysis of zedoarondiol-treated HBSMCs revealed significant enrichment of differentially expressed proteins in cell proliferation-related pathways ...
我们的研究结果显示,SNI显著提高了脊髓小胶质细胞中CTRP9和AdipoR1的水平。此外,鞘内注射重组CTRP9(rCTRP9)可显著缓解SNI引起的机械性痛觉过敏和热痛觉反应。另一方面,rCTRP9通过影响SNI小鼠脊髓AMPK/NF-κB机制,介导小胶质细胞从促炎性M1状态向抗炎性M2状态的表型转换。此外,使用AdipoR1 siRNA或AMPK特异性拮抗剂...
Cav1 siRNA treatment resulted in >80% inhibition of Cav1 in both SKOV3-ip1 and HeyA8 OC cells (Supplementary Fig. 5 c). PDGFRβ levels increased upon treatment with Cav1 siRNA compared to CTL siRNA (Supplementary Fig. 5 d). These results confirmed that increased PDGFRβ levels upon miR...
6a, b). Then, primary neurons transfected with Cav-1 siRNA or control were treated with OGD and incubated with DiI-labeled EVs derived from HUVECs. We found that transfection of Cav-1 siRNA resulted in obvious reduction of DiI-EVs in neurons as determined by MFI (Fig. 6c). The level ...
techniqueculture,andCAV1·targetedsmallinterferingdouble—strandedRNAs(siRNA)wereintroducedintoMCF一 7cellsbyLipofectamineTM2000 .ADMresistancewasdetectedwithtrypanblueexclusiontesting.CAV1mRNAand proteinlevelsweredeterminedbyreversetranscription—polymerasechainreaction(RT-PCR)andWestemblot.The ...
3.取N}玎LFs第3.7代使用,由上海吉玛制药技术有限公司设 计、制备得到3个不同序列的正常人caVl.siRNA,再将实验分为7 组,分别为空白对照组(N组)、TGF—p1(10ng/m1)处理组(M组)、 Co—siRNA转染组、Co.siIⅢA+TGF.p1(1 Ong/m1)处理组、 cavl一siRNA转染组、cavl-siRNA+TGF.p1(10ng/m1)处理组、 ...