本研究发现七氟烷暴露后,海马神经元感受到氧化应激的刺激,Caspase-8被活化,并激活和裂解GSDME,裂解GSDME释放其活性N端结构域在细胞膜上打孔,表现为七氟烷暴露12 h时,细胞体变大、细胞连接疏松、细胞内水分增多;海马神经元七氟烷暴露24 h时,细胞核和细胞体明显变大、细胞膜破裂,且发生海马神经元树突突触变短、轴突延...
两篇文章的重要性在于,首次阐清了Yersinia感染后引起细胞死亡的通路,发现抑制TAK1活性可以通过Caspase-8对GSDMD发生活化,并且该过程不依赖于对GSDMD有更大酶活性的Caspase-1/11。值得一提的是,邵峰老师课题组在2017年曾解析Caspase-3可以...
两篇文章的重要性在于,首次阐清了Yersinia感染后引起细胞死亡的通路,发现抑制TAK1活性可以通过Caspase-8对GSDMD发生活化,并且该过程不依赖于对GSDMD有更大酶活性的Caspase-1/11。值得一提的是,邵峰老师课题组在2017年曾解析Caspase-3可以通过酶切GSDME分子激活焦亡【12】。这些研究进一步论证凋亡Caspase与炎症Caspase在...
与对照组血清相比,寿胎丸含药血清能够上调HTR-8/SVneo细胞miR-29c-3p表达,而Caspase-8,Caspase-3,GSDME活性及IL-1β,IL-18表达均显著下调.与溶剂对照组相比,寿胎丸治疗组小鼠胚胎吸收减少,胚胎吸收率显著降低,小鼠胎盘组织中miR-29c-3p显著升高,Caspase-8,Caspase-3,GSDME活性及血清中IL-1β,IL-18表达...
The functions of pyroptosis have become a hot spot in cancer research. The Shao group found that GSDME-mediated pyroptosis is associated with the side effects of chemotherapy. Following treatment with chemotherapeutic drugs, activated caspase-3 cleaves GSDME to induce pyroptosis in GSDME-expressing cell...
Synthetic lethality of combined ULK1 defection and p53 restoration induce pyroptosis by directly upregulating GSDME transcription and cleavage activation through ROS/NLRP3 signaling Wei Chen Kai-Bin Yang Xuan Li Journal of Experimental & Clinical Cancer Research (2024) Multiomics characterization of pyro...
asc-/-小鼠,nlrc4-/-和gsdme-/-小鼠是通过将体外翻译的cas9mrna和grna共显微注射到c57bl/6j的受精卵而获得。grna载体中的用于nlrc4小鼠的靶向序列为5′-cagacgagcccttattcaa-3′(seqidno.01),用于asc小鼠的靶向序列为5′-tatgggcgcatcccacgcg-3′(seqidno.02)。用于gsdme小鼠的靶向序列为5′-ttcgccttt...
Caspase 3/GSDME-dependent pyroptosis contributes to chemotherapy drug-induced nephrotoxicity. Cell Death Dis. 2021;12:186 pubmed publisher Mondal T, Shivange G, Tihagam R, Lyerly E, Battista M, Talwar D, et al. Unexpected PD-L1 immune evasion mechanism in TNBC, ovarian, and other solid ...
viruses. 2022 aug 15;14(8):1782. pmid: 36016404 3. jingying wu, siming lin, et al. "tnf-α contributes to sarcopenia through caspase-8/caspase-3/gsdme-mediated pyroptosis." research square. september 23rd, 2022. 4. xin ming, huan chen, et al. "porcine enteric coronavirus pedv ...
Abstract 2998: Inhibition of MDM2-p53 interaction by alrizomadlin (APG-115) induces pyroptotic cell death in gasdermin E (GSDME)-expressing cancer cells Mouse double minute-2 (MDM2)-p53 inhibitor alrizomadlin (APG-115) is an investigational agent known to induce apoptosis of TP53-wild type...