while tight control of innate immunity is essential for proper immune induction and balance. Here, we reported that apoptotic induction orcaspase-3overexpression caused dramatic reduction of differently triggered cytokine signalings in human cells, murine primary cells and mouse model, while the loss...
[4]Bcl-2 overexpression attenuates resveratrol-induced apoptosis in U937 cells by inhibition of caspase-3 activity.Park JW,Choi YJ,Suh SI,et al.Carcinogenesis.2001 [5]Food-derived polyphenols inhibit pancreatic cancer growth through mitochondrial cytochrome C release and apoptosis.Mouria M,Gukovskaya ...
GreenNuc™活细胞Caspase-3活性检测试剂盒(GreenNuc™ Caspase-3 Assay Kit for Live Cells)是一种使用新型的具有细胞膜通透性的Caspase-3/7绿色荧光底物来检测活细胞中Caspase-3/7活性的试剂盒,可用于实时检测活细胞的凋亡情况。 Caspase (Cysteine-requiring Aspartate Protease)是一个在细胞凋亡过程中起重要作用的...
摘要 细胞是一个复杂的有机整体，能够完成一系列生命活动。调控细胞衰老和程序性死亡的细胞凋亡过程，在其生命活动中占有重要地位。细胞凋亡主要由Caspase蛋白酶家族所调控，其中 Caspase-3起到关键作用。该文就C a s p a s e家族的构成、功能以及Caspase-3的特性和作用机制进行综 述。关键词 调亡；C a s p ...
1.1106cells/ml增殖的JurkatT细胞加入Camptothecin,Camptothecin终浓度为4-6M。 2.细胞37C孵育4小时。 2 试验用品: 1.1275mm的Falcon管 2.PBS缓冲液 3.凋亡检测试剂盒 Product PE Active Caspase-3 Apoptosis Kit 包括:PE-conjugated Polyclonal Rabit Anti-Active Caspase-3 Antibody Cytofix/CytopermSolution Perm/Wash...
将Jurkat细胞同时接种于一个96孔板中(Costar),80,000 cells/90μL/孔。用喜树碱(终浓度为20μM)处理Jurkat细胞,未处理组作为空白对照,处理时间为5小时。处理组和空白对照组加入或不加caspase 3/7抑制剂AC-DEVD-CHO (5μM)处理10分钟。每孔加入100μL caspase 3/7检测缓冲液,室温孵育1小时。使用NOVOStar...
tosisofasciteshepatomacells(AH 一130)inrelationtoMito- chondrialmembrancepotential[J].CellStructFunct,1997,22 (5):555—563. [2O]ParkJA,LeeKY,OhYj,eta1.Activationofcaspase 一3pro- teaseviaaBel——2——insensitivepathwayduringtheprocessofgin- senosideRh2 一inducedapoptosis[J].CancerLett,...
nitric oxide toxicity in islet cells[J].J Biol Chem,1 995,270 (19):l 1176--1 1180. [1 8】Meier HL,Millard C,Moser J.Poly (ADP —ribose) polymerase inhibito rs regulate th e mechan ism of sulfur mustard -initiated cell death in human lymphocytes[J].J Appl Toxicol,2000,20 ...
用途:Colorimetric substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase. 纯度:99 % 产地:中国/上海 品牌:沪震生物 包装规格:5mg 货号:HZ-71003 ...
A member of this family, caspase-3, has been identified as a key mediator of apoptosis in neuronal cells. Recent studies in snail, fly and rat suggest that caspase-3 also functions as a regulatory molecule in neurogenesis and synaptic activity. In this study, in addition to providing an ...