1. 合理的凋亡诱导处理方式:设置药物诱导时间和浓度梯度预实验,以确定特定细胞系中药物诱导 caspase-3 活化的最佳条件,并用 staurosporine #9953(1μM for 3hours)或 etoposide #2200(25μM for 5 hours)处理 HeLa,NIH/3T3 或 C6 cells 作为 cleave...
while tight control of innate immunity is essential for proper immune induction and balance. Here, we reported that apoptotic induction orcaspase-3overexpression caused dramatic reduction of differently triggered cytokine signalings in human cells, murine primary cells and mouse model, while the loss...
GreenNuc™活细胞Caspase-3活性检测试剂盒(GreenNuc™ Caspase-3 Assay Kit for Live Cells)是一种使用新型的具有细胞膜通透性的Caspase-3/7绿色荧光底物来检测活细胞中Caspase-3/7活性的试剂盒,可用于实时检测活细胞的凋亡情况。
1. 合理的凋亡诱导处理方式:设置药物诱导时间和浓度梯度预实验,以确定特定细胞系中药物诱导 caspase-3 活化的最佳条件,并用 staurosporine #9953(1uM for 3hours)或 etoposide #2200(25 uM for 5 hours)处理 HeLa,NIH/3T3 或 C6 cells 作为 cleaved caspase-3 的阳性参照。 2. 贴壁细胞发生凋亡后会变得难贴...
《Levistolide A synergistically enhances doxorubicin?induced apoptosis of k562/dox cells by decreasing MDR1 expression through the ubiquitin pathway》 作者:Yuanyuan Ding Weibo Niu Tao Zhang Jue Wang Jiao Cao He Chen Ruiqi Wang Hongli An 期刊:Oncol. Rep. 影响因子:3.041 PMID:30483812 ...
GreenNuc™活细胞Caspase-3活性检测试剂盒(GreenNuc™ Caspase-3 Assay Kit for Live Cells)是一种使用新型的具有细胞膜通透性的Caspase-3/7绿色荧光底物来检测活细胞中Caspase-3/7活性的试剂盒,可用于实时检测活细胞的凋亡情况。 Caspase (Cysteine-requiring Aspartate Protease)是一个在细胞凋亡过程中起重要作用的...
[4]Bcl-2 overexpression attenuates resveratrol-induced apoptosis in U937 cells by inhibition of caspase-3 activity.Park JW,Choi YJ,Suh SI,et al.Carcinogenesis.2001 [5]Food-derived polyphenols inhibit pancreatic cancer growth through mitochondrial cytochrome C release and apoptosis.Mouria M,Gukovskaya ...
Caspase-3、6和7都属于执行Caspase，它们不象启动Caspase，不能被募集到或结合起始活化复合体，而是依赖...
贝博® BBcellProbe® Caspase-3 活性检测试剂盒(Caspase 3 Activity Assay Kit)是采用比色光度法法检测细胞或组织裂解液中Caspase 3酶活性或纯化的Caspase 3酶活性的试剂盒。 Caspase(Cysteine-requiring Aspartate Protease)是一个在细胞凋亡过程中起重要作用的蛋白酶家族。Caspase 3也称CPP32、Yama或apopain,有时...
A member of this family, caspase-3, has been identified as a key mediator of apoptosis in neuronal cells. Recent studies in snail, fly and rat suggest that caspase-3 also functions as a regulatory molecule in neurogenesis and synaptic activity. In this study, in addition to providing an ...