(1992- )ꎬ女ꎬ河南焦作人ꎬ在读硕士ꎬ主要从事生物技术与工程研究ꎮ通讯作者:欧阳乐军(1977- )ꎬ男ꎬ湖南益阳人ꎬ教授ꎬ博士ꎬ硕士生导师ꎬ主要从事植物分子生物学研究ꎮ基于 Cas9~Free 的拟南芥 ALB3 基因编辑载体构建及验证马铭赛 1ꎬ2ꎬ3 ꎬ布梁灏 3 ꎬ陈自银 3 ꎬ黄嘉玲 3 ...
Here, we report a simple technique that allows the isolation of Cas9-free mutants, eliminating the need for outcrossing or other intricate methods. This method is based on the ability of Basta-sensitive Arabidopsis thaliana seedlings, which generally perish, to recover and grow...
The CRISPR/Cas9 genome-editing system has emerged as a popular powerful tool for biological research. However, the process of selecting efficiently edited Cas9-free plants is usually laborious and time consuming. Here, we demonstrated P2A to be the most efficient self-cleaving peptide for fusing ...
Mutations generated by CRISPR/Cas9 in Arabidopsis (Arabidopsis thaliana) are often somatic and are rarely heritable. Isolation of mutations in Cas9-free Arabidopsis plants can ensure the stable transmission of the identified mutations to next generations, but the process is laborious and inefficient. He...
dCas9-SSAP,无切割(Cleavage-Free)基因敲入系统 基因编辑是基因组和细胞工程的强大工具。以CRISPR–Cas为例,基因编辑可能导致DNA损伤并触发DNA修复过程,而这些过程往往容易出错。当改变长序列时,这种不想要的突变和安全问题可能会更加严重。作者将微生物单链退火蛋白(SSAP)与无催化活性的dCas9偶联用于基因编辑。这种无...
本文主要介绍美国斯坦福大学丛乐课题组在《Nature Cell Biology》杂志上发表的《dCas9-based gene editing for cleavage-free genomic knock-in of long sequences》论文,该文章介绍了一种新型的基因编辑工具—dCas9-SSAP。 基因编辑是基因组和细胞工程的强大工具。以CRISPR–Cas为例,基因编辑可能导致DNA损伤并触发DNA...
北京大学天然药物及仿生药物国家重点实验室、基础医学院病原生物学系鲁凤民、王杰科研团队探索了CRISPR/Cas9系统是否可以通过外泌体传递其基因编辑功能,并对其机制进行了初步探索。外泌体作为一种重要的细胞与细胞间物质和信息传递的工具,在多种生理和病理过程中都发挥着非常重要的作用,被广泛应用于诊断和治疗领域。基于前...
The CRISPR/Cas9 system is a powerful, revolutionary tool for genome editing. However, it is not without limitations. There are PAM-free and CRISPR-tolerant regions that cannot be modified by the standard CRISPR/Cas9 system, and off-target activity impede
transgene-freeSince the first successful completion of genome‐based editing in the model plant Arabidopsis using CRISPR/Cas9 in 2013 (Jiang et al., 2013), a large number of agronomic trait‐related genes have been successfully edited in rice, including leaf color‐related genes OsYSA, CAO1, ...
Towards DNA-free CRISPR/Cas9 genome editing for sustainable oil palm improvement 2024, 3 Biotech Transcriptional engineering for value enhancement of oilseed crops: a forward perspective 2024, Frontiers in Genome Editing View all citing articles on Scopus ...