Hongmei ZhangZhengjian YanMelissa LiMichael PeabodyTong-Chuan He基因与疾病(英文)Zhang H, Yan Z, Li M, Peabody M, He TC. CRISPR clear? dimeric Cas9-FoK1 nucleases improve genome-editing specificity. Genes Dis. 2014; 1:6-7.
SaCas9替代传统的dead SpCas9,降低SpCas9空间位阻对Fok1相互结合的影响,从而提高其打靶效率;dead SaCas9‑Fok1系统需要配合2个21nt的Sa‑sgRNA共同作用,Sa‑sgRNA长于Sp‑sgRNA(20nt),且SaCas9识别的PAM序列为6nt,长于SpCas9的PAM(3nt),所以当dead SaCas9‑Fok1配合两个Sa‑sgRNA使用,进一步降低了...
Small molecule-triggered Cas9 protein with improved genome-editing specificity. Nat Chem Biol. 2015; 11:316-318.10.1038/nchembio. 1793 [PubMed: 25848930]Zhang H, Yan Z, Li M, Peabody M, He TC. CRISPR clear? dimeric Cas9-FoK1 nucleases improve genome-editing specificity. Genes Dis. 2014...
pdead SaCas9Fok1重组质粒利用dead SaCas9替代传统的dead SpCas9,降低SpCas9空间位阻对Fok1相互结合的影响,从而提高其打靶效率;dead SaCas9Fok1系统需要配合2个21nt的SasgRNA共同作用,SasgRNA长于SpsgRNA(20nt),且SaCas9识别的PAM序列为6nt,长于SpCas9的PAM(3nt),所以当dead SaCas9Fok1配合两个SasgRNA使用,...