[5]Adli M. The CRISPR tool kit for genome editing and beyond. Nat Commun. 2018;9(1):1911. Published 2018 May 15. doi:10.1038/s41467-018-04252-2 [6]Lino CA, Harper JC, Carney JP, Timlin JA. Delivering CRISPR: a review of the challenges and approaches. Drug Deliv. 2018;25(1):...
[4]Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014;346(6213):1258096. doi:10.1126/science.1258096 [5]Adli M. The CRISPR tool kit for genome editing and beyond. Nat Commun. 2018;9(1):1911. Published 2018 May 15. doi:10....
中文名称 Cas9(CRISPR相关蛋白9)ELISA检测试剂盒 英文名字 Cas9 (CRISPR Associated Protein 9) ELISA Kit 供应商 Cell Biolabs 产品货号 PRB-5079 产品报价 ￥7400/96assays 产品说明书 点击查看 购买方式 产品新闻 背景资料 产品描述 产品特点 保存建议 Upon receipt, aliquot and store the Cas9 Standard ...
1.a)在Opti-MEM无血清培养基中(ThermoFisher Scientific)加入合成的靶向PPIB基因外显子2的crRNAs和tracrRNA(Dharmacon),室温孵育10 min,从而形成靶向PPIB基因的gRNA。使用TransIT-X2® Dynamic Delivery System (Mirus Bio) 或TransIT®-mRNA Transfection Kit ...
除此之外，安捷伦还提供一款更加便利的SureGuide CRISPR/Cas Complete Kit,（货号 5190-7714），这一款包含了体外sgRNA合成和Cas9核酸酶等所有的配套试剂，咱们只需要设计好sgRNA就可以愉快的做实验啦。接着安捷伦2100生物分析仪再次出马，用来验证目的基因酶切效率，检验是否敲除成功。这一次咱们可以选取High sensitive DNA...
质粒提取试剂盒QIAGEN Plasmid Midi Kit和胶回收试剂盒QIAEX II Gel Extraction Kit购自QIAGEN公司,Lipofection 3000购自ThermoFisher Scientific公司,BsmBⅠ-v2内切酶和T4 Ligase购自New England BioLabs公司,TCRP1抗体购自Novus Biologicals公司,...
TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein are designed to work with TrueGuide synthetic gRNAs, but can also be used with in vitro transcribed gRNA from the GeneArt Precision gRNA Synthesis Kit. 引用和文献 (14) 搜索 引用和文献 Abstract Highly efficient RNA-guided genome editing in ...
[6] Adli, M. The CRISPR tool kit for genome editing and beyond.Nat. Commun. 9, 1911 (2018). [7] Van Overbeek, M. et al. DNA repair profiling reveals nonrandom outcomes at Cas9-mediated breaks.Mol. Cell63, 633-646 (2016).
are able to meet most of the requirements for use in a variety of plants under normal or complex conditions. These methods also allow researchers to customize their own gRNA modules and to assemble multiple gRNA expression cassettes for multiplex genome editing. Using this kit, we found that CRI...
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