complex that is essential for spacer acquisition and determine the 2.3-Å-resolution crystal structure of the Cas1–Cas2 complex. Mutations that perturb Cas1–Cas2 complex formation disrupt CRISPR DNA recognition and spacer acquisitionin vivo. Active site mutants of Cas2, unlike those of Cas1,...
Mutations that perturb Cas1-Cas2 complex formation disrupt CRISPR DNA recognition and spacer acquisition in vivo. Active site mutants of Cas2, unlike those of Cas1, can still acquire new spacers, thus indicating a nonenzymatic role of Cas2 during immunity. These results reveal the universal ...
在一项新的研究中,来自美国加州大学伯克利分校的研究人员发现Cas1-Cas2如何找出它们将病毒DNA片段插入到细菌基因组中的这个位点,这样Cas1-Cas2随后就能够识别这种病毒DNA片段和发起攻击。相关研究结果于2017年7月20日在线发表在Science期刊上,论文标题为“Structures of the CRISPR genome integration complex”。 Cas1-...
Structural coordination between active sites of a Cas6-reverse transcriptase-Cas1--Cas2 CRISPR integrase complex CRISPR-Cas systems provide adaptive immunity in bacteria and archaea by targeting foreign DNA for destruction using CRISPR RNA-guided enzymes. CRISPR immunity begins with integration of ...
This process is driven by foreign DNA spacer (termed protospacer) selection and integration mediated by Cas1-Cas2 protein. Recently, different states of Cas1-Cas2, in its free form and in complex with protospacer DNAs, were solved by X-ray crystallography. In this paper, molecular dynamics ...
在一项新的研究中,来自美国加州大学伯克利分校的研究人员发现Cas1-Cas2如何找出它们将病毒DNA片段插入到细菌基因组中的这个位点,这样Cas1-Cas2随后就能够识别这种病毒DNA片段和发起攻击。相关研究结果于2017年7月20日在线发表在Science期刊上,论文标题为“Structures of the CRISPR genome integration complex”。
Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity DOI号: https://doi.org/10.1073/pnas.1616395114 关键词: Cas2/3,Cas1,CRISPR I-F 1.背景 CRISPR有6种型(type I-VI),19种亚型。Type I是最常见的一种类型,有7种亚型(subtype I-A to I-F,and I-U),这7中亚型都...
The 3D structure of several snapshots of Type II-A Cas1–Cas2 complex during integration indicated that the catalytic Cas1 subunit inserts an α-helix into the minor groove of the leader duplex (28). The location of this α-helix is conserved in other Cas1–Cas2 structures (26, 29), ...
Chem. Commun. (Camb.) (8) , 886-7, (2002) We have successfully prepared an unsymmetrical analogue of a Katsuki-type salen ligand having a single hydroxyalkyl group at its 6-position, and also its Mn(III) complex; attachment of the complex to ... 详情 1...
英文名称:Borane-2-picoline complex CAS编号:3999-38-0 别名:(2-甲基吡 啶-1-鎓-1-基)三氢化 硼 纯度:98% 分子式:C6H10BN 产地:郑州 品牌:阿尔法 用途:科研实验 郑州汇聚(阿尔法)化工是集开发、生产、销售为一体的高科技公司。公司拥有一批长期从事精细化学品开发和生产的 技术人员,以及设备齐全的研发实验...