Movie-Freak Opinions of a Movie Freak I'm Peter, production year 1966 and living in Belgium. I'm married with Veronika and together we have two lovely kids , Karolien (7y) and Filip (5y). I'm a big movie-fan. I've been watching movies all my life and lost count of how many I...
With the electric bike system motor activated, you could haul 2 fat twin boys who are the same age as Dale and Brennan in the classic movie Step Brothers. 400 pounds is going to be a breeze to carry with the electric bicycle motor. The tires might be flat-resistant, but I would still...
LPS induces neutrophil and platelet adhesion to the mesentery (Supplementary Movie 1); representative still images of adherent neutrophil-platelet aggregates from an LPS-only control mouse are shown in Fig. 4b (left). Platelets (red) decorate the surface of adherent Ly6G+ neutrophils (blue), ...
Full size image Still, we aimed to show the cargo flow more directly and co-expressed mApple-Rab5, GFP-SNX1 and Halo-tagged TfR in mammalian cells. We detected transfer of TfR from Rab5 to SNX1-positive compartments (Fig. 5e and Supplementary Movie 15). Similarly, repeating this experimen...
He had an 8mm movie camera which he took various shots of and he took a couple of shots of the Waiben coming into the main wharf and once when my grandmother was a passenger on the Waiben which I think she boarded in Townsville and returned on it again on its next round trip. The...
a, Integrated and motion corrected micrograph of FHF complexes from movie collected using a K3 camera in counting mode (1.09 Å/pixel). Data collection repeated three times with either isolated FHF (two datasets) or bound to KIF1C stalk (one dataset, where KIF1C stalk did not alter th...
For the AP-1:Arf1:tetherin-Nef (L2A) sample, movie data were processed using MotionCor2-1.0.0 (Zheng et al., 2017) and the contrast transfer function of full dose non-weighted micrographs was estimated using Gctf-v1.06 (Zhang, 2016). 162,309 particles were picked from non-dose-weighted...
For full tilt series, this results in an accumulated dose of 104e− Å−2. Tomogram Reconstruction Tomogram reconstruction was handled with the TOMOMAN pipeline.58 First, motion correction was performed with MotionCor2,59 with each EER movie split into 40 fractions. Averages from the odd ...
(with a shape smoothing function), these puncta were tracked across the length of the movie using the ‘Object Tracking’ function. The GFP-objects were overlayed to the mCherry channel, and the average red fluorescence was measured for each object per frame. The threshold of what constitutes...
Cellular cargos move bidirectionally on microtubules by recruiting opposite polarity motors dynein and kinesin. These motors show codependence, where one requires the activity of the other, although the mechanism is unknown. Here we show that kinesin-3 K