a, Integrated and motion corrected micrograph of FHF complexes from movie collected using a K3 camera in counting mode (1.09 Å/pixel). Data collection repeated three times with either isolated FHF (two datasets) or bound to KIF1C stalk (one dataset, where KIF1C stalk did not alter th...
Full size image Still, we aimed to show the cargo flow more directly and co-expressed mApple-Rab5, GFP-SNX1 and Halo-tagged TfR in mammalian cells. We detected transfer of TfR from Rab5 to SNX1-positive compartments (Fig. 5e and Supplementary Movie 15). Similarly, repeating this experimen...
Previous studies showed that purified full-length p62 can also form helical filaments7,8. The existence of these assembly structures inside of cells, however, had not been demonstrated. Therefore, we used the CLEM technique to identify and visualize the ultrastructural organization of p62 found in ...
Transport at the molecular scale is a prerequisite for the development of future molecular factories. Here, we have designed oligoanionic molecular sliders on polycationic tracks that exploit Brownian motion and diffusive binding to transport cargo witho
Supplementary Movie S8 Rights and permissions This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the ...