[18F]FDG Cardiac Sarcoidosis Prep: high-fat and low-carbohydrate diet 24 hours prior [18F]FDG scan and at least over 18 hours of fasting, intravenous administration of heparin (50 unit/kg)122 Inflammation and viability Viability Prep: 6-12 hours of fasting. Oral glucose load+insulin or...
Tbx5 and Pitx2 form an incoherent feed-forward loop that is driven by Tbx5 and suppressed by Pitx2, governing a left atrial transcriptional network that regulates rhythm effector gene expression43. Reduced Tbx5 expression in adult mice disrupts the expression of calcium handling and AF-susceptibi...
doi:10.1101/757294Jakub TomekCold Spring Harbor Laboratory
The iPSC-CMs showed sarcomeric structures and cardiac calcium cycling. Gene expression manipulation by AAV6 Full differentiated iPSC-CMs were transduced with AAV6 particles. A multiplicity of infection (MOI) of 1 × 103 was used for the AAV6-mock (AAV6-GFP; Catalog No. 7008, Vector Biolabs,...
cardiac myoblast cell line to HOCl resulted in a dose-dependent increase in necrotic cell death, which could be prevented by SeMet supplementation and was attributed to SeMet preventing the HOCl-induced loss of mitochondrial inner trans-membrane potential, and the associated cytosolic calcium ...
Inhibition of the mevalonate pathway ameliorates anoxia-induced down-regulation of FKBP12.6 and intracellular calcium handling dysfunction in H9c2 cells. J. Mol. Cell. Cardiol. 2015, 80, 166–174. [Google Scholar] [CrossRef] [PubMed] Yang, Y.; Rong, X.; Lv, X.; Jiang, W.; Yang, Y....
If the ER stress is maintained, the ER leaks calcium, which could damage the mitochondria, thereby generating an excess of free radicals and promoting the activation of apoptosis, in turn generating cell death and tissue injury [18]. In previous studies from our group, we evaluated the role ...
cardiac myoblast cell line to HOCl resulted in a dose-dependent increase in necrotic cell death, which could be prevented by SeMet supplementation and was attributed to SeMet preventing the HOCl-induced loss of mitochondrial inner trans-membrane potential, and the associated cytosolic calcium ...