解释carbohydrate-binding module family 43是什么: CBM43(carbohydrate-binding module family 43)是属于碳水化合物结合模块(Carbohydrate-Binding Modules, CBMs)家族的一个成员。CBMs是一类在生物体内广泛存在的蛋白质结构域,它们能够特异性地识别和结合糖类分子。CBM43作为其中的一种,具有独特的结构特征和生物学功能,在...
Carbohydrate-binding moduleSoluble expressionLevan-agaroseEscherichia coliProteins with novel functions or advanced activities developed by various protein engineering techniques must have sufficient solubility to retain their bioactivity. However, inactive protein aggregates are frequently produced during ...
Green, signal peptide; blue, N-terminal glycoside hydrolase family 5 catalytic module; red, immunoglobulin-like module; orange, C-terminal family 46 CBM. EXPERIMENTAL PROCEDURES Carbohydrates All carbohydrates were purchased from Megazyme International (Bray, County Wicklow, Ireland), except hydroxyethylce...
Kilburn, A family 2a carbohydrate-binding module suitable as an affinity tag for proteins produced in Pichia pastoris, Protein Expres. Purif. 21 (2001) 417-423.Boraston, A. B., B. W. McLean, M. M. Guarna, E. Amandaron-Akow, and D. G. Kilburn. 2001. A family 2a carbohydrate-...
Ahmed, S., Louis, S. A., Bras, L. A. J., Fontes, C. M. G. A. and Goyal A. (2013). The family 6 carbohydrate binding module (CtCBM6B) of Clostridium thermocellum alpha-L-arabinofuranosidase binds xylans and thermally stabilized by Ca2+ ions. Biocatal. Biotransform. In Press....
Family 92 carbohydrate-binding modules specific for β-1,6-glucans increase the thermostability of a bacterial chitinase. Biochimie 212, 153–160 (2023). Article CAS PubMed Google Scholar Jam, M. et al. Unraveling the multivalent binding of a marine family 6 carbohydrate-binding module with ...
Family 92 carbohydrate-binding modules specific for β-1,6-glucans increase the thermostability of a bacterial chitinase. Biochimie 212, 153–160 (2023). Article CAS PubMed Google Scholar Jam, M. et al. Unraveling the multivalent binding of a marine family 6 carbohydrate-binding module with ...
1. Family 42 Carbohydrate-Binding Modules (CBM42s) bind the arabinose side-chains of complex hemicelluloses. 2. CBM42s display a beta-trefoil fold, which comprises 3 sub-domains designated as α, β and γ. 3. Each one of the three sub-domains presents a putative carbohydrate-binding pock...
Family 1 carbohydrate-binding modules (CBMs) are ubiquitous components of multimodular fungal enzymes that degrade plant cell wall polysaccharides and bind specifically to cellulose. Native glycosylation of family 1 CBMs has been shown to substantially impact multiple physical properties, including thermal ...
Endoglucanase Cel5A from Clostridium josui contains a family 17 carbohydrate-binding module (CBM) (CjCBM17) and a family 28 CBM (CjCBM28) in tandem. These two CBMs bound to non-crystalline cellulose and -1,3-1,4-glucan. Our results indicate that the CBMs recognized different components on...