近期发表在《Blood》期刊上题为“In vivo CAR T-cell generation in nonhuman primates using lentiviral vectors displaying a multidomain fusion ligand”的一篇文章研究了一种VivoVec的慢病毒系统,该慢病毒通过在T细胞结合时同时激活T细胞受体复合体和共刺激分子,模仿了生理上关键的启动信号,从而体内生成了大量高质量...
Umoja对VivoVec进行了系统的临床前试验,结果显示T细胞共刺激分子可以增强VivoVec粒子与T细胞结合、活化与转导,促使生成更多CAR-T细胞并增强体内抗肿瘤活性,MDF设计还可以可大幅降低给药剂量,提高安全性。 Umoja在2024年6月11日的Blood杂志上在线发表了标题为“In vivo CAR T-cell generation in non-human primates usin...
除此之外还需要进行淋巴细胞清除化疗来支持CAR-T细胞植入,往往会导致严重的不良反应。因此,科研人员尝试开发直接在体内进行基因工程改造,并生成CAR-T细胞的方法,希望能克服自体CAR-T细胞治疗的限制。 2024年6月,《blood》发表了题为“In vivo CAR T-cell generation in non-human primates using lentiviral vectors di...
Nicolai CJ, Parker MH, Qin J, et al. In vivo CAR T-cell generation in nonhuman primates using lentiviral vectors displaying a multidomain fusion ligand. Blood. 2024;144(9):977-987. doi:10.1182/blood.2024024523. 识别微信二维码,添加抗体圈小编,...
3.Hu Y,Mei H,et al.2062 Bio-Orthogonally Redirected Bispecific Lentiviral Vectors for In Vivo CAR-T Cell Generation. 4.Hu Y. The risk factors for coagulation disorder of chimeric antigen receptor-T cell therapy in pat...
3.Charitidis FT, Adabi E, Thalheimer FB, Clarke C, Buchholz CJ. Monitoring CAR T cell generation with a CD8-targeted lentiviral vector by single-cell transcriptomics. Mol Ther Methods Clin Dev. 2021 Oct 5;23:359-369. doi: 10.1016/j.omtm.2021.09.019. Erratum in: Mol Ther Methods Clin...
CAR T cellsChimeric antigen receptor (CAR) T cells are in prime focus of current research in cancer immunotherapy. Facilitating CAR T cell generation is among the top goals. We have recently demonstrated direct generation of human CD19-CAR T cells by targeting CD8 cells using lentiviral vectors...
今年7月，Nature杂志发文表示，来自临床和临床前研究的证据强调了CAR - T疗法在肿瘤以外的应用潜力，比如在治疗自身免疫、慢性感染、心脏纤维化、衰老相关疾病和其他疾病方面的潜力。2023年9月， Nature子刊“nature reviews drug discovery”上发表了一篇影响因子为120.1（Q1 IF=120.1），名为“The next-generation...
simultaneously knock out the endogenous TCR, which will eliminate GvHD concerns and allow for an allogenic T-cell product to be generated. Combining the CAR T-cell manufacturing process with simultaneous TCR knockout allows for a streamlined approach to universal CAR T-cell generation(Fig.4b, c)...
CAR-T cell generation by lentivirus The CAR sequence was cloned into the pCDH lentiviral vector backbone containing an EF1α promoter. Lentivirus was produced by transfecting 293T cells with CAR plasmid, pMD2.G and psPAX2 using polyethylenimine. Virus-containing supernatants were collected after 3...