Generation process of CAR-NK cells (Cancer Sci. 2021 Sep;112 基于CAR-T的设计策略,通过设计针对不同肿瘤抗原靶点的CAR,也使NK细胞能特异性的杀伤肿瘤细胞,如NK92-CD19-CD3ζ针对B系血液恶性肿瘤、CD138-CAR-NK-92针对多发性骨髓瘤等。目前,全球CAR-NK细胞疗法的开发和临床试验正在开展中,绝大多数CAR-NK...
CAR-T(CAR-γδT)细胞制备 T Cell Transduction process (TCT) CliniMACS Prodigy LP-3-56 System + PD-56 System CliniMACS Prodigy LP14-MoDc Process 1.从PBMC中分选出CD14+的单核细胞 2.对分选出的单核细胞进行培养扩增并定向分化为巨噬细胞 3.分化获得的巨噬细胞可用于接下来的基因修饰步骤 CliniMAC...
Trogocytosis is an active process that transfers surface material from targeted to effector cells. Using multiple in vivo tumor models and clinical data, we report that chimeric antigen receptor (CAR) activation in natural kil...
such as improved functionality of final iPSC-CAR-NK products. For example, gene editing can be applied to achieve better differentiation, expansion, and killing, improve resistance to the oppressive immune microenvironment and migration, which will process the application of iPSC-CAR-NK therapy in tr...
7. Tumor-infiltrating lymphocytes: Streamlining a complex manufacturing process. Emily Hopewell, et al. Cryotherapy, 11/4/2018 8. Large-scale expansion ofVγ9Vδ2T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimerlc antigen receptor-modified effector cells. Lin ...
CAR-T and CAR-NK Cell Therapies Ritwika Biswas, Field Scientist at Sino Biological, walks us through the chimeric antigen receptor (CAR) development process and how T and natural killer (NK) cells can be utilized to carry these modular synthetic molecules. Ritwika also addresses the safety and...
Integrated Approach: Support across the entire drug development process, integrating CAR-T mouse models and CAR-NK models with broader immuno-oncology services for efficient timelines. Our dedicated team ensures your therapies are tested with precision, helping you move closer to clinical success. App...
Production of large amounts of functional NK and CAR-NK cells represents one of the bottlenecks for NK-based immunotherapy. In this study, we developed a largescale, reliable, and practicable NK and CAR-NK production using G-Rex 100M bioreactors, which depend on ...
To further investigate the mechanism by which CAR-NK cells exert their cytotoxic effects on H1299 cancer cells, a high-content imaging system was utilized to document the dynamic killing process of both NK92 and dtCAR-NK92 cells as they interacted with H1299 cells. When H1299 tumor cells we...
To further investigate the mechanism by which CAR-NK cells exert their cytotoxic effects on H1299 cancer cells, a high-content imaging system was utilized to document the dynamic killing process of both NK92 and dtCAR-NK92 cells as they interacted with H1299 cells. When H1299 tumor cells we...