2.Roddie Claire et al. Manufacturing chimeric antigen receptor T cells: issues and challenges[J]. Cytotherapy, 2018, 21(3) : 327-340. 3.Mansour Poorebrahim et al,Production of CAR T-cells by GMP-grade lentiviral vector...
2.Roddie Claire et al. Manufacturing chimeric antigen receptor T cells: issues and challenges[J]. Cytotherapy, 2018, 21(3) : 327-340. 3.Mansour Poorebrahim et al,Production of CAR T-cells by GMP-grade lentiviral vectors: Latest advances and future prospects,CRITICAL REVIEWS IN CLINICAL LABORA...
低分化的初始样T细胞(naïve-like T cells ,TNcells,表型CD45RA+ CD45RO- CD95-,CCR7+,CD62L+,CD28+,CD27+)和干细胞记忆样T细胞(Stem cell memory-like T cells ,TSCMcells,表型CD45RA+ CD45RO- CCR7+CD95+)具有更强的增殖能力和自我更新能力,以及分化为效...
2.Roddie Claire et al. Manufacturing chimeric antigen receptor T cells: issues and challenges[J]. Cytotherapy, 2018, 21(3) : 327-340. 3.Mansour Poorebrahim et al,Production of CAR T-cells by GMP-grade lentiviral vectors: Latest advances and future prospects,CRITICAL REVIEWS IN CLINICAL LABORA...
为了在体外产生CAR-T细胞,慢病毒载体(LVs)由于其可以稳定的将大片段DNA有效的整合进分裂和非分裂细胞而被广泛应用。本文将综述生产CAR-T细胞的最新进展和挑战,以及用于制备GMP级别CAR-T 的LVs的生产过程,概述CAR-T治疗的应用进展,特别强调下一代同种异体CAR-T细胞。
2、Poorebrahim M, Sadeghi S, Fakhr E, Abazari MF, Poortahmasebi V, Kheirollahi A, Askari H, Rajabzadeh A, Rastegarpanah M, Linē A, Cid-Arregui A. Production of CAR T-cells by GMP-grade lentiviral vectors: latest advances and future prospects. Crit Rev Clin Lab Sci. 2019 Sep;56(...
CAR-T cellsThe clinical success achieved using CD19-directed CAR-T cells has stimulated many academic institutions to explore the feasibility of manufacturing these, and other CAR-T cells, in-house. This article reviews the issues that must be addressed in order to achieve this goal. It ...
(Christine Gagliardi, Mariam Khall & Aaron E Foster, Streamlined production of genetically modified T cells with activation, transduction and expansion in closed-system G-Rex bioreactors,Cytotherapy,2019;21:1246-1257) 首先,作者对传统的转导病毒培养模式和G-REX培养模式做了流程的对比...
Start the CAR: Constructing CAR-T Cells Production Pipeline of Chimeric Antigen Receptor T Lymphocytes Efficiency and Quality: Manufacturing CAR-T Cells for the Clinic Interested In Learning More? Complete to download FREE resource * Please enable Cookies and JavaScript before filling in the form. ...
近日,华南理工大学王均教授和许从飞副教授团队在Science Bulletin发表了题为“In vivoproduction of CAR-T cells using virus-mimetic fusogenic nanovesicles”的研究论文,提出了一种利用膜融合型纳米囊泡递送CAR蛋白在体构建CAR-T细胞的方法。 为了在体内制备CAR-T细胞,已有研究采用纳米颗粒来递送核酸物质,如质粒和mRNA...