This paper deals with the synthesis of m 3 2,2,7 G 5′ pppAmpUmpApd(CpTpTpApCpCpT), a 5′-TMG-capped RNA–DNA chimeric oligonucleotide, which is expected to be conveyed to the nucleus by snurportin 1, a nuclear-transport protein. This 5′-TMG-capped RNA–DNA chimeric molecule was ...
The resultant RNA is subjected to RNase I digestion and purification. Complete second strand cDNA synthesis with modified 5′ linkers and cleave double-stranded cDNA by EcoP15I. After ligation with 3′ linker, the cDNA library is purified with streptavidin beads. 1. First strand cDNA synthesis:...
It is important to note that mitochondria of budding yeast lack enzymes necessary for NAD synthesis and primarily rely on NAD imported from the cytoplasm42. In addition, the availability of free NAD dictates levels of mitochondrial NAD-capped RNA synthesis22. However, the full extent of Xrn1-H...
Next, we aimed to confirm the existence of these 3′UTR capped RNAs using independent methods. First, we asked if these fragments could be identified in transcriptomic (RNA-seq) data. For this we compared the CAGE signal with the RNA-seq signal of two different cell lines (Additional file ...
(4–7 nucleotides) for theArenaviridaeendonuclease [23]. These short-capped RNAs are subsequently used as primers for viralmRNA synthesisby the viral polymerase. By using this strategy, viruses kill two birds with one stone: de-capping of cellular mRNA blocks the expression of cellular RNA ...
Infection of cells by foot-and-mouth disease virus (FMDV) results in the rapid inhibition of host cell protein synthesis. This process is accompanied by th... GJ Belsham,GM Mcinerney,R Smith-N - 《Journal of Virology》 被引量: 0发表: 2000年 Eucaryotic initiation factor 4B controls eIF3-...
However, the synthesis of several adenovirus... José Luis Castrillo,Luis Carrasco - 《Journal of Biological Chemistry》 被引量: 46发表: 1987年 Influenza A virus RNA-dependent RNA polymerase: analysis of RNA synthesis in vitro. Influenza A virus RNA-dependent RNA polymerase, purified from virion...
专利名称：Preparation and isolation of 5′ capped MRNA 发明人：Anilkumar Kore,Shanmugasundaram Muthian,Irudaya Charles 申请号：US13324724 申请日：20111213 公开号：US08344118B2 公开日：20130101 专利内容由知识产权出版社提供 专利附图：摘要：The synthesis of capped/tagged RNA, methods of use and kits ...
Mechanism of Intraparticle Synthesis of the Rotavirus Double-stranded RNA Genome Rotaviruses perform the remarkable tasks of transcribing and replicating 11 distinct double-stranded RNA genome segments within the confines of a subviral ... KM Guglielmi,SM Mcdonald,JT Patton - 《Journal of Biological...
脱帽致意(cap的过去式与过去分词形式); 双语释义 n.(名词) [C]便帽; 帽子a soft flat covering for the head that has a curved part sticking out at the front as a peak and is worn especially as part of a uniform [C]保护盖〔套〕a protective covering for the end or top of an object ...