综合两篇文章对测序结果的分析,我们发现植物细胞中的NAD-RNA具有以下特征: (1) 大部分是细胞核基因的转录产物,也有小部分由线粒体基因组编码,这与酵母以及人类细胞中NAD-RNA的构成类似; (2) 植物NAD-RNA含有poly(A)尾巴,主要由蛋白质编码基因转录而来,也有少量的非编码基因的产物;(3) 植物细胞的叶绿体基因组...
Capped RNA refers to messenger RNA (mRNA) molecules that have a modified nucleotide cap at the 5′ end, which is essential for stability and efficient translation of the mRNA into proteins. AI generated definition based on: Methods in Enzymology, 2007 ...
Once a nascent host cappedRNAis bound by influenzaRdRPit is cleaved by theendonucleasedomain at the N terminus of the PA subunit. The 3′ end of the resultingcapped RNAfragment is used to primeviral transcription. For this to occur, the 3′ end of the capped RNA fragment must translocate ...
帽状类似物:GTP经优化设计为1:4,zui大化转录生成RNA和帽状RNA所占比例。20ul的反应体系加入1µg DNA模板在2h内可以体外合成15-35 µg 7-甲基鸟苷帽状RNA,其产量是传统体外转录反应的10-50倍。 优势: 1)一步法直接合成带帽状结构的RNA,其结构与大多数真核生物体内的mRNA结构相同; 2)试剂盒内酶混合液...
(blue lines) of RNA-seq relative to 3′UTR CAGE peaks in K562 cells (Additional file 2: Table S1—ENCSR545DKY). Bottom: Schematic representation of paired-end read positioning. Forward and reversed paired-end reads are presented in yellow and blue, respectively, with the intensity of the ...
A method is provided for making synthetic capped RNAs. These compounds serve as substrates for the virally encoded endonuclease associated with influenza virus. We are able to assay for this unique and specific viral activity of cleavage of a capped RNA in vitro. Therefore, screening of ...
第二部分为单中心、双盲、随机化、安慰剂对照研究,旨在研究NMN对健康人群NAD-capped RNA转录组的影响。纳入40名健康受试者,随机分成两组服用NMN或者安慰剂进行血液中NAD-capped RNA转录组的测定。 国内首个三甲医院开展的NMN临床试验揭盲 去除标签后,NMN组20例,安慰剂组16例、脱落4例。相较于安慰组,NMN组血中NMN...
The RNA-binding protein AUF1 binds AU-rich elements in 3'-untranslated regions to regulate mRNA degradation and/or translation. Many of these mRNAs are pre... - 《Nucleic Acids Research》 被引量: 0发表: 2013年 Combinatorial mRNA binding by AUF1 and Argonaute 2 controls decay of selected targ...
Xrn1 is a deNADding enzyme modulating mitochondrial NAD-capped RNA Sunny Sharma, Jun Yang, Ewa Grudzien-Nogalska, Jessica Shivas, Kelvin Y. Kwan & Megerditch Kiledjian Nature Communications volume 13, Article number: 889 (2022) Cite this article 7403 Accesses 19 Citations 6 Altmetric...
NAD-capped RNA is first chemo-enzymatically biotinylated with high efficiency, permitting selective capture on streptavidin beads. Then, a highly efficient library preparation protocol tailored to immobilized, 5′-modified RNA is applied, with adaptor ligation to the RNA's 3′ terminus and reverse ...