Binding of eIF-4E to the 5'm<7>G cap structure of eukaryotic mRNA signals the initiation of protein synthesis. In the present invention, the binding site cap-binding domain of eIF-4E was identified as the region containing the sequence Trp<113> to Arg<122>. Thus, in a first preferred ...
Figure 5 shows the lac promoter sequence with the CAP binding site and the operator O1. The lac promoter is activated by the presence of the dimeric CAP activator protein, complexed with cAMP at the CAP binding site. The CAP protein binds weakly to the α-subunit of RNA polymerase.46 The...
Transcription factors (TFs) identification and binding site prediction The PlantRegMap tool was used to predict transcription factors (TFs)100. Promoter sequences, defined as the 1500 bp upstream flanking regions, were retrieved through EnsemblPlants v. 45 using the BioMart tool, based on the Morex...
we observed reduced expression of the transcription factor TGACG MOTIF-BINDING FACTOR 6 (TGA6; BaRT2v18chr1HG033440), which belongs to the basic leucine zipper (bZIP) gene family. TGA6 contains a TFBS in the promoter sequence ofREM4.1, thus potentially regulatesREM4.1....
Sequence alignments of the NCBP2 and NCBP3 RRM domains showed that the residues critical for NCBP1 binding in NCBP2 are not conserved in NCBP3 (Supplementary Fig. 5b). Consistent with this finding, yeast two-hybrid data indicated that NCBP3 binds NCBP1 through its unstructured C-terminal ...
The efficiency of trimethylation is reduced by deleting the Sm binding site from U2 RNA, but it is not altered by other mutations in the coding sequence. Insertion of artificial Sm binding sites either into a mutant U2 from which the natural binding site has been deleted or into SP6 ...
Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site. Yeast. 1995;11:681–9. Article CAS PubMed ...
ApplicationOnlySequence ApplicationPrivate ApplicationRole ApplicationRoleError ApplicationRoleWarning ApplicationRoot ApplicationWarning ApplyCodeChanges ApplyStyle ArchitectureExplorer ArcPie ArcRing ArcSegment AreaChart ArrangeSelection ArrangeShapes ArtboardSplit ASerif ASMFile ASPFile ASPGenericHandlerFile ASPRazorFile...
DNA sequence determinants for binding of the Escherichia coli catabolite gene activator protein. The consensus DNA site for binding of the Escherichia coli catabolite gene activator protein (CAP) is 22 base pairs in length and is 2-fold symmetric: 5'-A... A Gunasekera,YW Ebright,RH Ebright -...
CBP80 Cap binding protein subunit 1/80 kDa nuclear cap binding protein cDNA Complementary DNA DNA Deoxyribonucleic acid E. coli Escherichia coli eIF4G Eukaryotic translation initiation factor 4 gamma EST Expressed sequence tags IRES Internal ribosome entry site I-TASSER Iterative Threading ASSEmbly Refine...