The highly conserved Cap Binding Complex (CBC) is comprised of two subunits, Cbp20 and Cbp80, and binds to the 5' end of pre-mRNA as it emerges from the transcribing RNA polymerase (Pol II). Evidence suggesting a role for the CBC in multiple reactions during gene expression, including ...
and co-ordinates multiple gene expressionevents.Key words: cap-binding complex (CBC), 7-methylguanosine cap(7mG), splicing, transcription, translation.INTRODUCTIONIn the eukaryotic cell, the maturation and translation of RNApol II (RNA polymerase II) transcripts into proteins requires aco-ordinated ...
Biochemistry Regulation of eukaryotic gene expression by mRNA cap binding complex and capping machinery SOUTHERN ILLINOIS UNIVERSITY AT CARBONDALE Sukesh R. Bhaumik LahudkarShwetaA characteristic feature of gene expression in eukaryotes is the addition of a 5' terminal 7-methylguanine "cap" to nascent ...
Demystifying the role of the Cap Binding Complex (CBC) within the gene expression network in Saccharomyces cerevisiae The highly conserved Cap Binding Complex (CBC) is comprised of two subunits, Cbp20 and Cbp80, and binds to the 5' end of pre-mRNA as it emerges from the tr... MB Clagget...
Cap-binding proteins specifically bind to the cap structure and mediate its functions in the cell. Two major cellular cap-binding proteins have been described to date: eukaryotic translation initiation factor 4E (eIF4E) in the cytoplasm and nuclear cap binding complex (nCBC), a nuclear complex ...
To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and do
CBC is a multifaceted complex essential for gene expression, which integrates RNA processing events, transcript nuclear export and translation. IDENTIFICATION OF CBC AS A NUCLEAR CAP-BINDING COMPLEX CBC was first purified from nuclear extracts of HeLa cells on the basis of its affinity for ...
To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the...
2.3. Cloning of CBP20 and CBP80 from B. juncea For partial cloning of nuclear cap binding complex proteins BjCBP20 and BjCBP80, primers (Table 1) were designed using the EST sequences obtained from Brassica Genome Gateway (http://brassica.nbi.ac.uk/). Using leaf cDNA as template, PCR...
Promoter-proximal pausing of RNAPII coincides with the formation of the cap structure at the 5′ end of pre-mRNA, which is bound by the cap-binding protein complex (CBC). Although the positive transcription elongation factor b (P-TEFb) stimulates the release of RNAPII from pausing and ...