www.nature.com/scientificreports OPEN Cell-free synthesis of functional human epidermal growth factor receptor: Investigation of ligand- received: 16 March 2016 independent dimerization in Sf21accepted:07September2016 Published:27September2016 microsomal membranes using non- canonical amino acids Robert B...
But for the polymer chemist, the twenty "canonical" amino acids provide an unacceptably small set of starting materials for macromolecular design. In the past several years, powerful techniques have been developed to introduce non‐natural (non‐canonical) amino acids containing a wide variety of ...
Amino acids are essential building blocks in biology and chemistry. Whereas nature relies on a small number of amino acid structures, chemists desire access to a vast range of structurally diverse analogues1,2,3. The selective modification of amino acid side-chain residues represents an efficient ...
The interaction of 11 amino acids with the TiO2 (101) anatase surface was investigated by means of PBE-D2* periodic simulations, both from a static and dynamic points of view. Several adsorption states, with the amino acid in its canonical, zwitterionic,
We demonstrate that itRNATy2 can initiate translation in vivo with aromatic nonヽanonical amino acids (ncAAs) bearing diverse sidechains. Although the initial system suffered from low yields, deleting redundant copies of tRNAfMet from the genome afforded an E. coli strain in which the efficiency...
Genetic code expansion (GCE) offers many exciting opportunities for the creation of synthetic organisms and for drug discovery methods that utilize in vitro translation. One type of GCE, sense codon reassignment (SCR), focuses on breaking the degeneracy
Orthogonal (O) ribosome-mediated translation of O-mRNAs enables the incorporation of up to three distinct non-canonical amino acids (ncAAs) into proteins in Escherichia coli (E. coli). However, the general and efficient incorporation of multiple distinct ncAAs by O-ribosomes requires scalable str...
Moreover, this amino acid should endow the aglycones with some specific interactions, thereby increasing the substrate binding affinity to a productive level. Thus, we first identified the amino acids within 4.0 Å away from the desosamine of either substrate, including Glu85, Glu94, Phe178...
02 September 2016 accepted: 29 November 2016 Published: 23 December 2016 Qinglei Gan1, Brent P. Lehman2, Thomas A. Bobik2 & Chenguang Fan1 The diversity of non-canonical amino acids (ncAAs) endows proteins with new features for a variety of biological studies and biotechnologica...
To overcome these limitations, we have developed a general, multiplexed method in which we genomically encode non-canonical amino acids (ncAAs) into multiple, structure-informed, individual sites within a target MBC; select for ncAA-containing MBC variants that assemble and function like the wild...