1National Bone Marrow Donor Program, Minneapolis, MN, USA; 2Be the Match, Minneapolis, MN, USA Abstract: Background: Hematopoietic cell transplant (HCT) offers a potential cure for life-threatening blood cancers, such as leukemia and lymphoma. HCT is a resource-intensive treatment, requiring pat...
The protein level of pro-inflammatory cytokines (TNFα and IL-6) in the MoNT, MoET, and MoLPSpost-culture and the co-culture experiment supernatants were analyzed with ELISA kits from R & D Systems (Minneapolis, MN, USA) according to the manufacturer’s protocols. Colourimetric changes in ...
TNFα concentrations in serum were determined by ELISA as recommended by the manufacturer (Quantikine, R&D Systems, Minneapolis, Minnesota). Zymography for MMPs was performed as described (Daniels et al., 2003). Statistical analysis The data were analyzed by unpaired Student's t test (2-tailed)...
The human phospho-kinase array kit (ARY003B) was obtained from R&D Systems (Minneapolis, MN). ELISA and lactate assays Media from cultured cells were collected and used for measuring IL-8 and lactate levels with Human IL-8 (Biolegend, San Diego, CA) ELISA kit42 and Lactate Colorimetric ...
The level of myeloperoxidase (MPO), IL-6, and IL-1β in the colonic tissues was measured using a mouse MPO enzyme linked immunosorbent assay (ELISA) kit (HK210, Hycult Biotechnology, The Netherlands), a mouse IL-6 Quantikine ELISA kit (R&D Systems Inc., Minneapolis, MN), and a mouse ...
A METAvivor Metastatic Translational Research Award (Minneapolis Metsquerade Presented In Memory of Tarah Harvey 2019, AB) was the primary funding mechanism for this study. Additional support provided by an NCI Cancer Center Support Grant (P30CA016672, MDA) and an Early Clinical Investigator Award...
Hirsch (PI), Jo Ann Jameson (PC); Hennepin County Medical Center, Minneapolis, Minn: Richard T. Zera (PI), Carol A. Schmidt (PC); Huntsman Cancer Institute at the University of Utah, Salt Lake City: John Harris Ward (PI), Chaunda L. Biggs (PC); Indiana Community Cancer Care, ...
The following results support the significance of laminin-1 in the invasion of CSCC7 cells: 1.) Tumor-associated fibroblasts produced more laminin-1 and less components of fibrillar ECM than normal cells; 2.) The production of laminin chains was further increased when CSCC7 cells were grown in...
Caspase-3 fluorogenic protease assay was performed according the manufacturer’s protocol (R&D Systems, Minneapolis, MN). In brief, 3 × 106 cells were lysed in 250 μl of cell lysis buffer, and total cell lysates were incubated with 50 μM of DEVD-AFC substrate for 1 h. Caspase-3 acti...
Reagents were purchased from the following suppliers: C75 (#2489) from Tocris Bioscience (Minneapolis, MN); ABT-263/navitoclax (#A3007) from APExBio (Houston, TX); ABT-199/venetoclax (#CT-A199) from ChemieTek (Indianapolis, IN); TVB-3166 (#SML1694) from Sigma-Aldrich (St. Louis, MO)...