a–cAssays were performed with RT2-cancer cells (a,b), RT2.Stat1−/−-cancer cells (a),Cdkn2a-deficient RT2-cancer cells (b), or RT2.CRISPR-Ctr- control, or RT2.CRISPR-Cdkn2a-cancer cells (c). For the senescence growth assay, cells were cultured either with medium (Ctr) or wi...
Upon anticancer treatment, cancer cells can undergo cellular senescence, i.e., the temporal arrest of cell division, accompanied by polyploidization and subsequent amitotic divisions, giving rise to mitotically dividing progeny. In this study, we sought to further characterize the cells undergoing sene...
To induce senescence in B16F10 melanoma cells, cells were treated with various concentrations of doxorubicin. Senescence-associated beta-galactosidase (SA-β-gal) staining showed that doxorubicin concentrations higher than 0.1 μM successfully induced senescence. A doxorubicin concentration of 0.1 ...
This plasticity can operate in several manifestations (Fig. 2). Thus, nascent cancer cells originating from a normal cell that had advanced down a pathway approaching or assuming a fully differentiated state may reverse their course by dedifferentiating back to progenitor-like cell states. Conversely...
Understanding the global metabolic changes during the senescence of tumor cells can have implications for developing effective anti-cancer treatment strategies. Ionizing radiation (IR) was used to induce senescence in a human colon cancer cell line HCT-116 to examine secretome and metabolome profiles. ...
Researchers from Shanghai Jiao Tong University's School of Medicine and the Netherlands Cancer Institute found they can selectively induce senescence in liver cancer cells with mutations in a gene named TP53. Senescence means the loss of a cell's power of division and growth, similar to putting ...
Abnormal O2 levels induce shortening of telomeres “culture shock” culturing condition of both human and mouse cells Oxidative stress, endoplasmic reticulum stress interferon (IFN)-related responses “therapy-induced senescence” (TIS) :Treatment with DNA damage agents such as UV,-irradiation , te...
induce p16INK4At 2. Therapy-induced senescence 大多是通过DNA damage 来进行老化诱导的 B. Noncell-Autonomous Regulation of Senescence in Cancer: Role of SASP SASP对于肿瘤的形成来讲是一把双刃剑 senescent cells can induce paracrine senescence in neighboring cells, thus acting as a barrier against...
This is very important for the metastasis of cancer cells in vivo and an important strategy to control the development of cancer cells. Senescence Growing evidence suggests that MYB is a potential candidate for the regulation of senescence, and inhibition of MYB expression plays an essential role ...
which instead activates VEGF to promote the proliferation of tumor cells18. The easiest way to reduce ROS is high degree of hypoxia. Nevertheless, only concepts related to oncogene, such as Ras, indirectly support that high degree of hypoxia may induce senescence in cancer cells, without clear ...