We show that specific secreted factors, induced in metastasis-associated endothelial cells (ECs), promote metastasis in mice by enhancing stem cell properties and the viability of cancer cells. Perivascular macrophages, activated via tenascin C (TNC) stimulation of Toll-like receptor 4 (TLR4),...
The 2004 World Health Organization (WHO) classification defined SCLC as “a malignant epithelial tumor consisting of small cells with scant cytoplasm, ill-defined cell borders, finely granular nuclear chromatin, and absent or inconspicuous nucleoli.” ...
Transcribed RNAs undergo various regulation and modification to become functional transcripts. Notably, cancer transcriptome has not been fully characterized at transcript resolution. Herein, we carry out a reference-based transcript assembly across >
Cancer stem cells are the malignant counterparts of normal somatic stem cells that can maintain quiescence, divide symmetrically for self-renewal, and divide asymmetrically to repopulate the tumor cell population within a tumor. AI generated definition based on:Fetal and Neonatal Physiology (Fifth Editio...
Cell viability assays HN6, SCC7 cells were seeded in 96-well plates (6,000 cells per well). Cancer cells were then cultured with indicated CM for 48 h and the CCK-8 assay was used to determine cell viability. CCK-8 reagent (NCM Biotech, China) was diluted 10 times with FBS-free me...
SI很容易根据IHC分辨crypt和villus结构,以及stem-diff axis;而colon则很难,只能看到crypt,大部分都是goblet cell; https://quizlet.com/306420982/digestive-large-intestine-histology-diagram/ https://quizlet.com/306354948/digestive-small-intestine-histology-3-diagram/ ...
1a). Genome-wide gene essentiality scores for DEPMAP cancer cell models (n = 897) were estimated by CERES24, which measures the essentiality of each gene relative to the distribution of effect sizes for common essential and nonessential genes within each cell line25. Because many genes do...
Workflow diagram Full size image Methods Datasets TCGA (https://cancergenome.nih.gov/) is a public repository of genomics data (e.g., gene expression) for cancer, and sometimes adjacent normal tissues. TARGET is a similar resource focused on childhood cancers. The GTEx project is a collection...
We found that patterns of cell cycle checkpoint deregulation were associated with the intrinsic molecular subtype of breast cancer cell lines. Specifically, the luminal B and basal-like cell lines harbored two molecularly distinct Gap 2/mitosis checkpoint defects (impairment of the decatenation Gap 2...
(Fig.1b). We examined the intersection of these two lncRNA profiles, and 15 lncRNAs were upregulated in both the cancer versus normal analysis and in the ER-negative versus ER-positive analysis, as shown in the Venn diagram (Fig.1cand TableS1). According to the prioritization of ...