Absence of FMR-1 gene expression can be detected with RNA extracted from dried blood specimens. Hum Genet 1994;93:488-93.Jih-Tung Pai,Shih-Feng Tsai,Chen-Jee Horng,Pao-Chin Chiu,Ming-Yuan Cheng,Kwang-Jen Hsiao,Kuang-Dong Wuu.Absence of FMR-1 gene expression can be detected with RNA ...
RNA was then extracted for qRT-PCR. RNA immunoprecipitation Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (Millipore) was used to accomplish RNA immunoprecipitation according to the manufacturer’s instructions. Cells were rinsed twice in ice-cold PBS before being lyzed with an equivalent ...
Microarray analysis Total RNA was extracted from snap-frozen livers samples from control and 36-h PhX livers. Briefly, frozen samples were homogenised using Precellys 24 ceramic mix bead tubes (Stretton Scientific Ltd) in a Precellys 24 homogeniser (Stretton Scientific Ltd) at 6500 rpm for 30 ...
Methods:HEU and HU infants were recruited from informal settlements of Cape Town. Stool DNA was extracted using MoBio PowerFecal DNA kit and 454 or Illumina sequencing was performed. Data was preprocessed using QIIME and UPARSE and imported into R for further analyses using the R package phyloseq...
However, three other groups of SOB are classified according their ability to use different electron donors and carbon sources (Lin et al., 2018; Pokorna and Zabranska, 2015; Tang et al., 2009): - Chemolithoheterotrophs (e.g., Beggiatoa and Thiobacillus): These bacteria gain energy from ...
They furthermore showed that the seeded barrier can be modulated by single-point mutations in the core domain or by interactions with the N-and C-terminal tails [100, 163]. It should be noted that in-vitro seeding is often assisted by a cofactor (RNA or heparin), which was recently ...
4.2. Preparation of gRNA and Cas9 mRNA The in vitro transcription of gRNA oligos was performed with a HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, E2040S, Ipswitch, MA, USA), in accordance with the manufacturer's protocol. The in vitro transcription of Cas9 mRNA ...
as single-cell RNA sequencing (scRNAseq) requires sequenced reads to be mapped and aligned to a single reference genome. Using theAstyanax mexicanus, this study highlights how the interpretation of a single-cell dataset from the same sample changes when aligned to its two different available genome...
Total RNA was extracted from TRIzol™ cell lysates using phenol-chloroform extraction. Isolated RNA was purified using GenElute™ Mammalian Total RNA Miniprep Kit (Merck, Darmstadt, Germany) and genomic DNA contamination was avoided by on-column incubation with DNase I (Merck). cDNA was prepared...
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