Absence of FMR-1 gene expression can be detected with RNA extracted from dried blood specimens. Hum Genet 1994;93:488-93.Jih-Tung Pai,Shih-Feng Tsai,Chen-Jee Horng,Pao-Chin Chiu,Ming-Yuan Cheng,Kwang-Jen Hsiao,Kuang-Dong Wuu.Absence of FMR-1 gene expression can be detected with RNA ...
Total RNA was extracted from plantlets grown in vitro from buds and seeds, which were used as template for One-step RT-PCR detection of the viruses. Results showed that viruses are present in both cultivars from the three collection orchards. Concentrations of amplicons varied among cultivars, ...
Viral RNA was extracted from plasma samples collected from five individuals during the period of viremia before seroconversion in primary infection with hu... LQ Zhang,P Mackenzie,A Cleland,... - 《Journal of Virology》 被引量: 574发表: 1993年 Determinants of entry cofactor utilization and tropi...
while MX-1/T cells with TPP+. At least 1 × 106cells were used for genomic DNA extraction using NucleoSpin Tissue Kit according to the manufacturer’s protocol (Macherey–Nagel, Germany). The total RNA was extracted from about 2 × 106cells with mirVana...
Ameliorating bone loss caused by mechanical unloading is a substantial clinical challenge, and the role of noncoding RNAs in this process has attracted increasing attention. In this study, we found that the long noncoding RNA osteoblast differentiation-r
Transport of lncRNA AL139294.1 by EVs and its role in NSCLC Because AL139294.1 was identified in serum EVs from patients with NSCLC, we hypothesised that it played an oncogenic role in recipient cells upon EV-mediated transport. To verify the hypothesis, we initially co-cultured Beas-2B cells...
RNA from T24 cells infected with sh-CIT (n = 3) or sh-Control lentivirus (n = 3) was extracted using Trizol reagent. The quantity of RNA was evaluated using NanoDrop. The integrity was measured by Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA). Only samples with ...
To determine the extent of the innate cellular immune response to transfected RNA, we monitored the release of a wide range of cytokines and chemokines into the culture medium, as well as the stability of protein expression from circRNA and cell viability. We found that RNase R digestion of ...
LncRNAs belong to the long non-coding RNA family of more than 200 nucleotides. Although most of them cannot code protein, some of them still have been proven to be able to be translated into some functional peptides [14]. According to the HUMAN GENCODE, more than 16,000 lncRNAs are reco...
This gene expression can be stable when the foreign DNA is inserted into the genome or maintained as stable episomes. Expression can also be transient, whereby the maintenance of DNA or RNA in the cells is temporary, and the expression is lost over time17. Stable genetic transformation of ...