Absence of FMR-1 gene expression can be detected with RNA extracted from dried blood specimens. Hum Genet 1994;93:488-93.Jih-Tung Pai,Shih-Feng Tsai,Chen-Jee Horng,Pao-Chin Chiu,Ming-Yuan Cheng,Kwang-Jen Hsiao,Kuang-Dong Wuu.Absence of FMR-1 gene expression can be detected with RNA ...
RNA was extracted by chloroform extraction, precipitated using isopropanol, washed with ethanol and resuspended in 20–30 µl of nuclease-free water. 2–5 µl of resuspended RNA was set aside to run on a gel and the remaining was DNase-treated in DNase buffer (100 mM Tris-HCl,...
Uterine fluid RNA extraction and sequencing To perform transcriptome sequencing, we collected 144 uterine fluid specimens from 48 participants and extracted total RNA using a commercial kit. As expected, the yield of RNA was relatively low, ranging from 0 to 1160 ng with an average of 148 ...
H.S. extracted RNAs and performed the qPCR assays. R.B. grew virus pools and titred virus infectivity in samples from in vitro and in vivo experiments. O.O.A. titred virus infectivity for in vitro experiments. D.V. and L.C.S.C. analysed the RNA-seq data. C.Z. acquired and ...
RNA sequencing of small RNA moleculesextracted from Francisella novicida U112 culture containing Cas12a-based CRISPRloci revealed that mature crRNAs for Cas12a protein are 42–44 nt in length, with thefirst 19/20 nt corresponding to the repeat sequence and the remaining 23-25 ntto the spacer se...
RNA was extracted from five different rat brain regions during development, starting from embryonic day 15 (E 15 ) until postnatal day 60 (P 60 ). These RNA preparations were analyzed by both Northern and dot blot for their content of 2... JN Kanfer,M Parenty,C Goujet-Zalc,... - ...
(I) RNA was extracted from G + P-resistant or -naive U87MG cells, and qRT-PCR was performed using primers for FGF1 and FGF2. (J) G + P-resistant or -naive U87MG cells were treated with 10 μM G + 3 μM P or 0.5 μM PD173074 (PD) for 4 h. Cell lysates were analyzed ...
RNA from T24 cells infected with sh-CIT (n = 3) or sh-Control lentivirus (n = 3) was extracted using Trizol reagent. The quantity of RNA was evaluated using NanoDrop. The integrity was measured by Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA). Only samples with ...
Cisplatin sensitive and resistant breast cancer cells were cultured with DMEM (10% FBS) and subsequently using TRIzol reagent, RNA was extracted following manufacturer’s protocol (Ambion, life technologies). Isolated RNA was then converted to complimentary DNA deploying RT-reagent kit (TakaRa). The...
RNA was then extracted for qRT-PCR. RNA immunoprecipitation Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (Millipore) was used to accomplish RNA immunoprecipitation according to the manufacturer’s instructions. Cells were rinsed twice in ice-cold PBS before being lyzed with an equivalent ...