Mouse Canx qPCR Primer Pair,即小鼠Canx qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一...
Human NCAN qPCR Primer Pair 一键复制产品信息 产品编号:QH12613S 产品包装:200次 选择包装 200次 数 量 价格:¥22.00
Cells were processed for qPCR 48 h post-infection. c) Fold change of DENV 2 genomic RNA levels in Vero cells prophylactically treated with Cas13a and the indicated guide from the virus only condition using each primer set. The bars represent the geometric mean of n = 2 independent ...
Evaluation of customized TqPCR primers that can be used to assess the RT-reverse transcription quality (RQ) of qPCR templates from mouse RNA samples.Qian, Zhang
RT–qPCR primers. A list of all of the RT–qPCR primers used in the included experiments listed. Source data Source Data Fig. 3 Source Data Fig. 4 Source Data Extended Data Fig. 6 Source Data Extended Data Fig. 9 Source Data Extended Data Fig. 10 Rights and permissions Reprints and ...
Reverse transcribed into cDNA using the HiScript II Q RT SuperMix for qPCR (+gDNA wiper) (Vazyme, China). All reactions were performed with ChamQTM SYBR Color qPCR Master Mix (Vazyme, China) on a Bio-Rad CFX96 Touch™ PCR machine (Bio-Rad, CA), using the primers shown in Table 1...
RNA for qPCR was extracted using the RNeasy kit (Qiagen, Germany). Reverse transcription was carried out using the High Capacity cDNA RT kit (Applied Biosystems, Life Technologies, USA). Following reverse transcription, qRT-PCR was performed using SYBR Green (Qiagen, Germany). beta-Actin (ActB...
Through bioinformatics analysis, the reads within each amplification family are compared and PCR artifacts and sequencing errors are removed to form a consensus sequence. Figure 2. The tags can also be used to detect errors and false positives; if they are inserted 'too early' in the strand, ...
Human CANX qPCR Primer Pair 一键复制产品信息 产品编号:QH11389S 产品包装:200次 选择包装 200次 数 量 价格:¥22.00
The abundance of transcripts was determined using the RT2 SYBR Green Rox Fast Mastermix (Qiagen) with an QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher Scientific). Data analysis was performed as described previously84. Sequence information for QPCR primers is available in Supplementary ...