CFTR Calu-3细胞 迁移采用常规培养肺腺癌细胞Calu-3的方法,利用MTT和CCK-8法确定囊性纤维化跨膜传导调节因子(cystic fibrosis transmembrane conductance regulator,CFTR)的激活剂(Genistein)和抑制剂(CFTRinh-172)的最适浓度,用CFTR激活剂和抑制剂的最佳浓度分别处理Calu-3细胞,然后采用划痕实验观测了培养48h后Calu-...
CFTRCalu-3细胞迁移采用常规培养肺腺癌细胞Calu-3的方法,利用MTT和CCK-8法确定囊性纤维化跨膜传导调节因子(cystic fibrosis transmembrane conductance regulator,CFTR)的激活剂(Genistein)和抑制剂(CFTRinh-172)的最适浓度,用CFTR激活剂和抑制剂的最佳浓度分别处理Calu-3细胞,然后采用划痕实验观测了培养48 h后Calu-3...
While the conclusions cannot be extrapolated to other airway epithelia, the present results demonstrate that mostHCO3−secretion by Calu‐3 cells is mediated by CFTR.doi:10.14814/phy2.13641Huang, JunweiKim, DusikShan, JiajieAbu‐Arish, Asmahan...
CFTR阳性的细胞株是Capan-1(ATCCHTB-79)。;细胞传代方法:消化5-10分钟。1:2。3天内可长满。;细胞生长特性:贴壁生长;细胞形态特性:上皮样;相关细胞有:ARPE-19Cells、Roswell Park Memorial Institute 6666Cells、H-7721细胞 细胞物种来源:人源或鼠源等其它物种来源 Calu-3 Cells|人肺腺癌克隆细胞(包送STR鉴定...
摘要: 采用常规培养肺腺癌细胞Calu-3的方法,利用MTT和CCK-8法确定囊性纤维化跨膜传导调节因子(cystic fibrosis transmembrane conductance regulator,CFTR)的激活剂(Genistein)和抑制剂(CFTRinh-172)的最适浓度,用CFTR激活剂和抑制剂的最佳浓... 查看全部>> ...
Calu-3 cells also express the wild- type CFTR protein,23 which allows for the examination of the relationship between CFTR and mucin regulation, especially with respect to altered mucin sulfation occur- ring in cystic fibrosis.24 Hoechst 33258 staining of nuclei The effects of different PEI ...
Protease-activated receptor regulation of Cl- secretion in Calu-3 cells requires prostaglandin release and CFTR activation. Human lung epithelial (Calu-3) cells were used to investigate the effects of protease-activated receptor (PAR) stimulation on Cl(-) secretion. Quantitative... ML Palmer,SY Le...
Presents a study which provided evidence to support the point that exocytosis is not involved in activation of chlorine (Cl-) secretion through cystic fibrosis transmembrane conductance regulator (CFTR) within the Calu-3 airway epithelial cells. What cystic fibrosis (CF) is caused by; Information on...
Exocytosis is not involved in activation of Cl- secretion via CFTR in Calu-3 airway epithelial cells. Am J Physiol Cell Physiol 275, C913-C920.Loffing J, Moyer BD, McCoy D, and Stanton BA. Exocytosis is not involved in activation of Cl- secretion via CFTR in Calu-3 airway epithelial ...
Here, we examined whether HCO3- may also regulate the expression of sAC and thereby affect the production of cAMP upon activation by HCO3- and the stimulation of CFTR in BCECs. Results RT-competitive PCR indicated that sAC mRNA expression in BCECs is dependent on [HCO3-] and incubation time...