Subculturing, also referred to as passaging, is the removal of medium and transfer of cells from a culture into fresh growth medium, in order to propagate the cells. See our product list for this protocol Brightfield image of Calu-3 cells in culture, prior to transfection....
ApplicationTransfection: Gene Expression 产品 货号产品名称规格单价 (CNY)数量 11668019Lipofectamine™ 2000 Transfection Reagent, 1.5 mLEach 6,152.00 Every step of the way, a wide range of cell health products Maintaining healthy cells is the key to experimental success and reproducible research results...
Gel shift analysis showed that IL-1β activated NF-κB in Calu-3 cells, and transfection experiments using p50 and RelA expressing vectors showed that exogenous transfected NF-κB subunits increased the concentration of CFTR mRNA. Gel shift analysis with antibody supershifting also showed that IL-...
Applications:transfection host (Roche FuGENE® Transfection Reagents) Tumorigenic:Yes Antigen Expression:Blood Type A; Rh+; HLA A10, A11, B15, Bw35 DNA Profile (STR):Amelogenin: X CSF1PO: 10 D13S317: 11,12 D16S539: 11 D5S818: 10,12 ...
Directions for useResuspend lyophilized shRNA plasmid DNA in 500 μl of deionised water. Each vial contains 50 μg of lyophilized shRNA plasmid DNA. Suitable for up to 50 transfections. Quality ControlThe sequence of shRNA is guaranteed by sequencing. ...
2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439. Applications: transfection host (Roche FuGENE® ...
TransfectionBlotting, WesternGenetic VectorsApoptosisCell ProliferationGene Expression Regulation, NeoplasticLoss of expression and p53 mutations are critical events in the early stages of lung carcinogenesis.The restoration of Fhit function in -negative cancer cells has been reported to cause tumour ...
使用Lipofectamine 3000 试剂转染 Calu-3 细胞 适当的培养技术和程序是确保转染成功的重要组成部分。传代培养(也称为传代)是指移除培养基并将培养物中的细胞转移到新鲜的生长培养基中,从而实现细胞增殖。 请参阅我们为本实验方案提供的产品列表 转染前培养物中 Calu-3 细胞的明场图像。
DNA 量(DNA 浓度应为 0.5–5 μg/μL) 250 ng P3000™ 试剂 0.5 μL 3 将管2溶液加入管1中,混匀 4 将步骤3所得混合物在室温下孵育 10-15 min 5 向细胞中加入 50 μL 步骤4所得复合物;轻轻涡旋平板,确保复合物均匀分布至整个孔中
4将步骤3所得混合物在室温下孵育 10-15 min 5向细胞中加入 50 μL 步骤4所得复合物; 轻轻涡旋平板,确保复合物均匀分布至整个孔中 转染效率分析 在转染 GFP 报告基因构建体后 48 h,通过显微镜和流式细胞仪评价细胞。为了评估转染效率,首先通过荧光显微镜观察细胞,以定性评估蛋白表达、形态和...