METHODS: We constructed a eukaryotic expression vector pcDNA3.1/cagA and a luciferase reporter vector pGL/gastrin promoter, and then co-transfected them into gastric cancer AGS and SGC-7901 cells. The two kinds of gastric cancer cells were, respectively, infected with cagA-positive H. pylori NCT...
The reporter activities were mea- sured with a dual luciferase assay kit (Promega) 24 h after infection of 60190 or isogenic strain of CagA deletion, then normalized with renilla activities. Reporter activities were presented as relative light units to those obtained from the negative control. ...
In this study, mutation of 1 of the 3 CAGA boxes located at 730, 580, and 280 of the PAI-1 promoter decreased the Ox-LDL-induced luciferase activity by 40 to 58%, whereas mutations in 2 sites reduced it over 75% or completely abolished it. Overexpression of Smad3 in N-terminal ...
用普通PCR的方法,琼脂糖凝胶电泳观察CagA对miR-155表达的影响.6,在GES-1细胞中瞬时转染miR-155-3p minic和miR-155-5p-minic,普通PCR检测其对KLF4 mRNA影响;通过转染不同的时间,不同的剂量,Western blot检测其对KLF4蛋白表达的影响;WT-KLF4 luciferase reporter和MUT-KLF4 luciferase reporter与miR-155 minic/NC...
AGS was transfected with two PAR1b-specific duplex small interference RNA (PAR1b-siRNA-535 and PAR1b-siRNA-709) or luciferase-specific siRNA (used as a negative control) in the presence or absence of CagA-ABD-HA. Cells were harvested at 36 h post-transfection, and PAR1b expression ...
The regulation of AUF1 on GKN1 was determined by RNA pulldown assay, RNA immunoprecipitation, mRNA turnover, and luciferase activity assays. The involvement of phosphorylated extra-cellular signal-regulated kinase (p-ERK) or CagA in H. pylori-induced AUF1 expression was verified using p-ERK ...
The regulation of AUF1 on GKN1 was determined by RNA pulldown assay, RNA immunoprecipitation, mRNA turnover, and luciferase activity assays. The involvement of phosphorylated extra-cellular signal-regulated kinase (p-ERK) or CagA in H. pylori-induced AUF1 expression was verified using p-ERK ...
Helicobacter pyloriluciferaseType IV secretion systemType IV secretion of effector proteins is an important principle for interaction of human pathogens with their target cells. The corresponding secretion systems may transport a multitude of effector proteins that have to be deployed in the respective ...
Additionally, MAB also suppressed the IL-8 luciferase activity and its secretion in HP-infected ACS cells. On the other hand, molecular structure simulations revealed that MAB interacts with CagA similarly to that of cholesterol. Moreover, binding of cholesterol to the immobilized CagA was inhibited...