CAF-1是基于其在SV40 DNA模板体外复制过程中促进核小体从头组装的能力而发现的。它结合新合成的H3.1-H4组蛋白,并与组蛋白H3-H4伴侣蛋白ASF1和复制过程因子增殖细胞核抗原(PCNA)合作,促进其在S期的复制叉上沉积。人的CAF-1对体内复制偶联染色质组装至关重要,但相应的酵母复合体的破坏不会明显影响细胞生长。这可能...
CAF-1的发现是基于其在体外复制SV40 DNA模板时具有促进新生核小体组装的能力。它结合新合成的H3.1-H4组蛋白,并与组蛋白H3-H4的分子伴侣ASF1和复制进程因子增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)协同作用,在S期促进组...
对人体静息细胞通过博来霉素(Bleocin)处理,发现CAF-1和其相互作用蛋白PCNA显著表达,CAF-1和PCNA被招募到受损的染色质中通过NER和NHEJ修复途径进行SSBs和DSBs的DNA损伤修复,再通过RNA干扰导致CAF-1的缺失,静息细胞活力显著下降、DSBs增加,说明CAF-1在人类静息细胞中发挥着修复DNA链断裂后重组染色质的作用[74]。 4 总...
CAF-1是基于其在SV40 DNA模板体外复制过程中促进核小体从头组装的能力而发现的。它结合新合成的H3.1-H4组蛋白,并与组蛋白H3-H4伴侣蛋白ASF1和复制过程因子增殖细胞核抗原(PCNA)合作,促进其在S期的复制叉上沉积。人的CAF-1对体内复制偶联染色质组装至关重要,但相应的酵母复合体的破坏不会明显影响细胞生长。这可能...
Hengstschlager M, Rosner M, Fountoulakis M and Lubec G. Regulation of PCNA and CAF-1 expression by the two tuberous sclerosis gene products. Biochem Biophys Res Commun 2003, 307: 737 -742.Markus Hengstschlager,Margit Rosner,et al.Regulation of PCNA and CAF-1 expression by the two tuberous...
CAF-1 large subunit is recruited to Spo11 and VDE meiotic DSB, independently of strand invasion and of its interaction with PCNA.Elsa, BrachetClaire, BéneutMariaElisabetta, SerrentinoValérie, Borde
The findings were correlated with the expression of proliferation cell nuclear antigen (PCNA) and patients' clinicopathological and follow-up data. CAF-1/p60 was expressed in all the tumours, whereas CAF-1/p150 was down-regulated in a number of cases. Overexpression of CAF-1/p60 and down-...
Abstract Genome stability in eukaryotic cells is maintained through efficient DNA damage repair pathways, which have to access and utilize chromatin as their natural template. Here we investigate the role of chromatin assembly factor 1 (CAF-1) and its interacting protein, PCNA, in the response of...
Use of nucleotide excision repair (NER)-deficient cells shows that the NER pathway—specifically dual incision—is required for recruitment of CAF-1 and PCNA. This in vivo demonstration of the local role of CAF-1, depending directly on NER, supports the hypothesis that CAF-1 ensures the ...
(西北农林科技大学动物医学院农业部动物生物技术重点实验室 , 杨凌 712100 )摘 要: 染色质组装因子 1 (chromatin assembly factor-1 , CAF-1), 是由 p150 、 p60 、 p48 三个亚单位组成的组蛋白 伴侣 ° CAF-1 主要功能是在 DNA 复制中与增殖细胞核抗原 (proliferating cell nuclear antigen , PCNA) ...