van Breemen RB, Li Y (2005) Caco-2 cell permeability assays to measure drug absorption. Expert Opin Drug Met 1(2):175-185van Breemen RB, Li Y. Caco-2 cell permeability assays to measure drug absorption. Expert Opin Drug Metab Toxicol. 2005;1:175-85....
Permeability across differentiated monolayers of CaCo-2 cells measured to estimate human intestinal permeability. Principle of the assay:●CaCo-2 cells are grown to confluence and allowed to differentiate on filters. Test agent is added to one side of the monolayer, and permeability is assessed ...
Before incubation, it is necessary to check the barrier integrity of Caco-2 cells, which is typically assessed using Transepithelial Electrical Resistance (TEER) or Lucifer Yellow Rejection Assay (%LY Leakage). Test compound permeability assay protocol ...
【3】Hubatsch I , Ragnarsson E G E , Artursson P . Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers[J]. NATURE PROTOCOLS, 2007, 2(9):2111-2119. 【4】 Delie F , Rubas W . A Human Colonic...
Please provide an overview of Cyprotex's Caco-2 Permeability assay. Caco-2 cells are widely used as an in vitro model for predicting human drug absorption. The Caco-2 cell line is derived from a human colorectal carcinoma, and when cultured, the cells spontaneously differentiate into monolayers...
2 cell monolayer permeability assays combines multi-well plates fitted with semipermeable inserts on which Caco-2 cells have been cultured with liquid chromatography-mass spectrometry (LC-MS) or LC-tandem mass spectrometry (LC-MS-MS) for the quantitative analysis of test compounds and the ...
笔者在本实验通过对细胞形态学特征、生长活力、跨膜电阻值(transepi-thelial electrical resistance,TEER)、荧光黄表观渗透系数(apparent permeability coefficient,Papp)和碱性磷酸酶活性等参数进行检测,评估和验证Caco-2细胞模拟小肠吸收的体外模型系统,建立可靠、稳定、重复性好的Caco-2细胞体外吸收模型,为本实验室后续...
Permeability coefficients across monolayers of the human colon carcinoma cell line Caco-2, cultured on permeable supports, are commonly used to predict the absorption of orally administered drugs and other xenobiotics. ICE Bioscience has the assay ready and ongoing for small molecules and PROTACs, alon...
Caco-2 Cell CultureEndocrine-Disrupting ChemicalsThe purpose of this study was to evaluate the permeability characteristics of endocrine disrupting chemicals utilizing epithelial monolayers of Caco-2 cells. The drugs tested in this study were bisphenol A (BPA), tert-octylphenol (tOP), tert-butylphenol...
•Caco-2permeability:20-23day/Passage30-45 •24-wellformattranswell:0.31cm 2 surfacearea •Donorconc:100µMincluding1%DMSO •A:300µLpH7.4/B:1200µLpH7.4Ringersbuffer •Directionality:ABandBA(N=4) •Donorsidesampling:20µLatbeginningandend(90min) •Receiversidesampling:100µ...