Emerging evidence suggests that intestinal permeability can be potentially enhanced through cocrystallization. However, a mechanism for this effect remains to be established. In this study, we first demonstrate the enhancement in intestinal permeability , evaluated by the Caco-2 cell permeability assay, ...
Advantages of Caco-2 Permeability Assay: Predictive Capability: Highly correlated with human intestinal uptake to predictin vitrodrug behaviorin vivo. Versatility: Great for studying passive diffusion as well as active transportation, drug effluxetc. ...
Caco-2 cell permeability assays to measure drug absorption. Caco-2 cells are a human colon epithelial cancer cell line used as a model of human intestinal absorption of drugs and other compounds. When cultured as a ... B Richard,V Breemen,Yongmei,... - 《Expert Opinion on Drug Metabolism...
Cyprotex's Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarized Caco-2 cell monolayers to predict in vivo absorption of drugs.
•Caco-2permeability:20-23day/Passage30-45 •24-wellformattranswell:0.31cm 2 surfacearea •Donorconc:100µMincluding1%DMSO •A:300µLpH7.4/B:1200µLpH7.4Ringersbuffer •Directionality:ABandBA(N=4) •Donorsidesampling:20µLatbeginningandend(90min) ...
Permeability coefficients across monolayers of the human colon carcinoma cell line Caco-2, cultured on permeable supports, are commonly used to predict the absorption of orally administered drugs and other xenobiotics. ICE Bioscience has the assay ready and ongoing for small molecules and PROTACs, alon...
ADME/Tox Cell Lines License 基本信息 NACRES NA.81 产品性质 质量水平 200 生物来源 human male colorectal tissue (Source disease: adenocarcinoma) 形式 liquid technique(s) drug transporter assay: suitablepermeability assay: suitable OMIM登记号 171050601107 application(s) ADME/TOX 储存温度 −196℃ Gene...
Cell-based in vitro models for intestinal permeability studies 3.3.4.1 Caco-2 model Since their isolation from a human colorectal carcinoma (Fogh, Orfeo, Tiso, & Sharkey, 1979), Caco-2 cells have been considered the “gold standard” assay to predict intestinal absorption (Hidalgo, 2001). Caco...
Caco-2 MRP2 inhibitor assessment Caco-2 MRP2 CDCF-DA MK-571 Caco-2 MRP2 substrate assessment / Chemical inhibition Caco-2 MRP2 CDCF-DA MK-571 Caco-2 permeability testing Caco-2 - -DescriptionMonolayers based on Caco-2 cells (a human colon carcinoma cell line) express a wide range of tra...
The apparent permeability coefficient (Papp, cm/s) values of compounds 2 and 9 were determined as 45.34 ± 4.67 and 33.17 ± 5.15 × 10(-6) cm/s by Caco-2 cell permeability assay.doi:10.1007/s12272-014-0353-1Kim, SoheeCollege of Pharmacy, Catholic University of Daegu, 712-702, Hayang...