The discovery that Ca ions play key roles in biology, not only as cations that contribute to the generation of currents and to the control of potential at the plasma membrane, but also in the regulation of intracellular functions and activities, stems from the pioneer experiments of Ringer in ...
近期,澳门科技大学中医药质量研究国家重点实验室Neher创新药物发现生物物理实验室罗婉君课题组在Redox Biology上发表了题为“Hyperoside alleviates toxicity of β-amyloid via endoplasmic reticulum-mitochondrial calcium signal transduction cascade in APP/PS1 double transgenic Alzheimer's disease mice”的研究论文,揭示...
9 Functional characterization and relative expression levels of wild-type CaSR and CaSR mutants. a, IP1-accumulation assays monitoring functional responses of CaSR WT (with or without pretreatment with PTX) and CaSR mutants to Ca2+, categorized based on different structural domains/regions. b, ...
In the nervous system, only one type of neuron–glial synapse is known to exist: that between neurons and oligodendrocyte precursor cells (OPCs), yet their composition, assembly, downstream signaling and in vivo functions remain largely unclear. Here, we address these questions using in vivo micr...
Cell-specific expression of high levels of human 5100P. in transgenic mouse brain is dependent on gene dosage. J Neurosci 1992; 12: 4337–4346. PubMed CAS Google Scholar Gerlai R, Friend W, Becker L et al. Female transgenic mice carrying multiple copies of the human gene for S10013 ...
In addition, experiments were conducted to determine the expression levels of the MCU and mNCE in the two mitochondria pools using western blot. Mitochondrial RCI was higher in the synaptic group compared to the non-synaptic group, which is consistent with past results. Ca 2+ uptake and ...
2C, cytosolic peak values: 3.19 ± 0.08 μM in vector transfected control cells, n = 17 vs. 3.27 ± 0.08 μM in wt parkin overexpressing HeLa cells, n = 14;). Similarly, ER Ca2 + levels were unaffected by wt parkin overexpression (Fig. 1D, ER Ca2 + levels: 282.5 ± 14.48 μM ...
Elevated Ca2+ levels in mice produce an enhancement of carbon clearance. On the basis of the time required for the initiation of such effects, it appears that this increased phagocytic activity is not the major factor in previously observed modifications of antibody formation by altered Ca2+ levels...
We first quantified the basal cytosolic Ca2 þ levels in yeast cells equipped with the Ca2 þ -dependent reporter protein aequorin at standard external [Ca2 þ ] of B1 mM in the culture medium. In this setting, heterologous expression of aSyn provoked an elevation of the cytosolic Ca2 ...
R.S. carried out the molecular biology, hippocampal stereotaxic injections and most of the slice experiments with help from A.D.J. and H.C. B.S.H. performed all of the cortical virus injections and cranial window implantations for thein vivoexperiments. B.S.H. and R.S. did thein vivo...