A-23187 也可用于平衡细胞内外 Mg2+ 浓度,使其适用于校准 Mg2+ 指示剂。 图1.A23187化学结构 A23187基础信息 产品名:A23187,Ca2+ 离子载体;钙离子载体 A23187; 英文名:Calcium Ionophore A23187;A-23187; Antibiotic A-23187 结构式:C[C@H]1CCC2([C@@H](C[C@H]([C@H](O2)C(C)C(=O)c3ccc...
Proc Natl Acad Sci U S A 2013;110:18543-18548.Tateno H, Krapf D, Hino T et al (2013) Ca2+ ionophore A23187 can make mouse spermatozoa capable of fertilizing in vitro without activation of cAMP-dependent phosphorylation path- ways. Proc Natl Acad Sci U S A 110:18543-18548...
直接用DMSO就行了 一般配成1000X 不好意思，追问一下，你一般处理细胞的浓度是多少？处理多长时间？谢谢...
1) Ca~(2+) ionophore A23187 Ca2+载体A23187 2) CA CA 1. Effect of Ca on Compressive Creep Behavior of AE41 Alloy; Ca对AE41合金压蠕变行为的影响 2. Effects of Sn and Ca Addition on Microstructural Stability of ZA62 Magnesium Alloy; ...
Niflumic acid也可以通过Ca2+ ionophore A23187在Ca2+对卵母细胞的渗透作用下抑制ICl(Ca),表明ICl(CA)的抑制是因为与Cl-通道的直接相互作用,而不是通过电压依赖性Ca2+通道干扰Ca2+进入。 Niflumic acid对去甲肾上腺素和咖啡因诱发的IO(Ca)具有抑制作用(IC50:6.6 μM),弱于自发电流引起的电流。Niflumic acid对...
10 mM Ca2+ containing the calcium ionophore A23187 to the Ca2+ free parasite culture increased YFP/CFP ratio from 1.36 (Ca2+ = 2.93 nM (median); minimum between 0–30 sec) to 2.66 (Ca2+ = 895.2 nM; maximum between 120–180 sec) in trophozoite stage ...
CaCl2 together with the Ca2+ ionophore A23187 have been shown to increase intracellular Ca2+ levels in D. discoideum cells (Reed and Lardy, 1972, Lydan and O'Day, 1988a, Baskar et al., 2000). Baskar et al. (2000), have shown that cytoplasmic Ca2+ levels in cells of untreated ...
Conversely, cell treatment with the Ca2+ ionophore A23187 rescued the egress-defective phenotype of a shlA mutant, consistent with a functional role for increase in cytosolic Ca2+ in the bacterial egress process [41]. ShlA expression is associated with damages of the bacterial containing vacuole ...
In cases where egg activation fails following ICSI, many clinics attempt artificial egg activation using Ca2+ ionophores such as A2318713. However, these treatments only elicit a single rise in Ca2+ that does not reflect the series of Ca2+ oscillations seen at fertilisation, and may be ...
In this paper we report the rapid phosphorylation of a cytosolic 100 kDa protein during stimulation of secretion from dispersed aggregates of parotid acinar cells with Ca(2+)-mobilizing secretagogues (carbachol, Substance P, ATP and the Ca2+ ionophore A23187). Phosphorylation was inhibited by rem...