在三个Ca2+离子的作用下，水母发光蛋白分子将腔肠素-h底物氧化为腔肠酰胺，释放二氧化碳并在469nm处产生具有峰值的闪光，通过VICTOR Nivo检测。由于GPCR激活后细胞内Ca2+浓度增加且相应的闪光产生速度非常快，因此该检测需要极快速的测量响应。VICTOR Nivo读板仪的分液器可以满足这一需求，它可以在进样后0.1秒从微孔...
既往有研究报道过腺相关病毒介导心肌肌浆网(Ca2+ -ATPase 2a, sarcoplasmic reticulum Ca2+ -ATPase,SERCA2a)基因转导治疗大鼠慢性心力衰竭。 发表在2021年8月《循环》杂志的一篇基础研究假设MYDGF参与了心脏对压力超负荷诱导心力衰竭的适应性...
For the functional analysis of ion channel activity, an artificial lipid bilayer suspended over microwells was formed that ruptured giant unilamellar vesicles on a Si substrate. Ca(2+) ion indicators (fluo-4) were confined in the microwells by sealing the microwells with a lipid bilayer. An over...
Ion channel expression by white matter glia: the O-2A glial progenitor cell. Neuron 1990; 4: 507–524. 6. Kettenmann H, Kirischuk S, Verkhratsky A. Calcium signalling in oligodendrocytes. Neurophysiology 1994; 26: 26–31. 7. Soliven B. Calcium signalling in cells of oligodendroglial ...
b STRING analyses of upregulated (not downregulated) genes in the NRXN1α+/− neurons showed association with “calcium ion transport” (red, 12 genes, FDR + 3.20E−03), “calcium ion transmembrane transport” (blue, 9 genes, FDR = 2.06E−02), “voltage-gated calcium ...
To ascertain the role of Ca2 þ in photoreceptor degeneration caused by loss of Pde6, it is necessary to measure this ion within the inner segment. We therefore generated here a double-transgenic zebrafish, transgenic (Tg; Tr2:tdTomato; TaCP:GCaMP3), in which the Ca2 þ indicator GCa...
"Ion Exchange of H+, Na+, Mg2+, Ca2+, Mn2+, and Ba2+, on Wood Pulp" Journal of Wood Chemistry and Technology, 26: 259-272 (2006).Rudie A W,Ball A,Patel N.Ion Exchange of H+ Na+, Mg2+, Ca2+, Mn2+, and Ba2+, on Wood Pulp.Journal of Wood Chemistry and Technology. ...
Fig. 3. Non-voltage gated (voltage insensitive) and voltage gated (voltage sensitive) Ca2 + channels working as ion channel complexes with KCa channels. SOCs; Store Operated Channels, ROCs; Receptor Operated Channels. a, d: positive feedback loop between KCa and Ca2 + channels, b, c: negat...
et al. The ion channel TRPV1 regulates the activation and proinflammatory properties of CD4+ T cells. Nat. Immunol. 15, 1055–1063 (2014). Article CAS PubMed PubMed Central Google Scholar Simeoli, R. et al. Exosomal cargo including microRNA regulates sensory neuron to macrophage ...
5 ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/ncomms4916 a 4.0 3.0 IP3df 2.0 Hep 6.0 n=6 *** 4.0 2.0 0.0 IP3df +Hep 1.0 0.0 0 c 4.0 10 20 Time (min) Ctr 3.0 Tg 30 b 8.0 Time to 25% (s) 6.0 IP3df Ion+ IP3df 4.0 Ion 2.0 IP3df lClT lCl1 0.0 0 d 2.0 5 10 15...