The methods comprise determining binding of the LAG-3 protein, fragment, derivative, or analogue to MHC class II molecules using bio-layer interferometry (BLI). Such methods can be used as a quality control assay in good manufacturing practice (GMP) grade production of such compounds. Probes ...
sh wing2bindingsites rcalcium 2.4 AnnexinB1 在膜联蛋白家族中的进化位置 真核生物膜联蛋白的无根进化树共分为 3 支 ( 图 4), 扁形动物的 Annexin B1 和节肢动物 (arthr p d) 为 1 支 , 新杆线虫 (nemathelminth)~ 植 物 (plant)~ 鸟类 ( rnithy) 和脊椎动物 (vertebrate) 为 1 支 , 原虫 ...
~ a + / ~ Eaxc~ha+nge, Ca2+ Binding, and Electrogenic Ca2+ Transport in Plasma Membranes of Human Placental Syncytiotrophoblast SIDDHARTH G. KAMATH AND CARL H. SMITH The Edward Mallinckrodt Department of Pediam'cs and Pathology, Washington University School of Medicine at St. Louis Children...
The consensus amino acid sequence for an EF-hand Ca2+-binding domain has allowed identification of more than 200 Ca2+-binding proteins from primary structures, many of them expressed in the central nervous system (Table 2.3). A central question concerns the biological, function of these proteins...
Considering that this domain is less conserved among subtypes, compared to, for example, the IP3-binding or TM domain [64], enhanced diversity of interaction partners may drive the acquisition of different phenotypes of mutated proteins, with a subset of these interactions disrupted. In this ...
Cofilin-1 is a small actin-binding protein which belongs to the actin-depolymerizing factor/cofilin (ADF/cofilin) family. Mammalian cells express three highly conserved members of this family: ADF, cofilin-1 and cofilin-2 [5]. All of them have distinct expression patterns that may overlap in ...
Both R4064–4210 and a peptide representing the calmodulin-binding region of RyR1 (R3614–3643) alter the Ca2+ dependence of (3H)ryanodine binding to RyR1, suggesting that they may both be interfering with an intramolecular interaction between amino acids 4064–4210 and amino acids 3614–3643 ...
Nucleotides and Ca 2+ binding to α-actin prepared from ordinary skeletal muscle of carp Cyprinus carpio was studied. When bound Ca 2+ was removed with ethylenediaminetetraacetic acid, carp α-actin denatured more rapidly than chicken α-actin. Kinetic studies of the denaturation process showed tha...
(2) Ca2+storage by the luminal Ca2+-binding proteins (histidine-rich Ca2+-binding protein, calsequestrin (CSQ), sarcalumenin and junctate); and (3) Ca2+uptakers: SERCA2a pumps for Ca2+reuptake. As proper Ca2+storage, release and reuptake are essential for normal cardiac function, any ...
The simulations were focused on obtaining the free energy profiles designed to clarify the possible calcium binding modes. In all considered cases, the calcium ion is coordinated by carboxyl oxygen atoms and water molecules exclusively. The results allowed for (i) determining the dentacy of calcium...