qRT-PCR分析进一步揭示了相对表达与转录组数据高度一致。 结论:这些数据为C2H2锌指转录因子基因家族在该物种中的进一步深入研究提供了坚实的基础,并为C2H2 ZFPs基因家族在C. teeta花发育中的作用提供了初步信息。 关键词:生物信息学,C2H2-锌指转录因子,全基因组鉴定,Coptis teeta Wall.,花发育 三、结果 Figure 1 ...
锌指蛋白根据其在保守结构域中的 C 和 H 残基的 数量和位置可分为以下几类:C2H2,C2HC,C2HC5, C2C2,C3H,C3HC4,C4,C4HC3,C6 和 C8[14].其中 C2H2 型锌指蛋白(C2H2-ZFPS)的研究最为广泛. 这些蛋白质中的锌指结构域包含 2 个 C 和 2 个 H, 其保守序列通常为 CX(2～4)CX12HX(3～5)H(其中...
GLABROUS INFLORESCENCE STEMS2(GIS2)和ZINC FINGER PROTEIN8(ZFPS)通过整合赤霉素和细胞分裂素信号控制拟南芥表皮细胞形成和发育.本研究克隆和分析了3个与GIS基因具有高度同源性的锌指蛋白基因ZINC FINGER PROTEIN5(ZFPS),ZINC FINGER PROTEIN6(ZFP6)和GLABROUS INFLORESCENCE STEMS3(GIS3),利用遗传学和分子生物学...
The C2H2 zinc finger proteins (ZFPs) play essential roles in regulating cold stress responses. Similarly, raffinose accumulation contributes to freezing stress tolerance. However, the relationship between C2H2 functions and raffinose synthesis in cold tolerance remains uncertain. Here, we report the chara...
植物C2H2型锌指蛋白的结构和功能.pdf,专论与综述 遗传HEREDITAY,(BP“z”H)26(3):414～418.2004 植物C2H2型锌指蛋白的结构与功能 黄骥,王建飞,张红生 南京农业^学作物遗传与种质创新国家蕈点实验窀,甫京210095) 摘要:锌指蛋白是一类具有指状结构域的转录因子。根据半
but the most numerous are the Kruppel-type C2H2 ZFPs. Many of these proteins contain either multiple tandem pairs of zinc-fingers or tandem arrays of three or more zinc-fingers. As transcription factors, they participate generally in the fundamental mechanism of gene expression. However, they usu...
The Q-type C2H2 ZFPs also contain two additional motifs, an L-box, rich with leucine residues and the B-box, containing a nuclear localization signal (NLS) required for transcriptional regulation. © The Author(s) 2018. This article is distributed under the terms of the Creative Commons ...
(non-classical nuclear location sequence, ncNLS),以協助鋅指蛋白進入細胞進行功用.本研究收集在前人研究中已被確認為ncNLS相關的10個鋅指蛋白,對其進行序列相關定位點分析,以探討鋅指蛋白和去氧核醣核酸的特異性結合點與鋅指蛋白中的非典型之核定位訊號位置之共同相關性.Zinc finger proteins (ZFPs) are a kind ...
大豆C2H2型锌指蛋白转录因子基因的克隆与鉴定论文 Abstract an member factorswhichexists The ofzinc is of family fingerproteinsimportant transcription in and all rolein and cukaryote.Zincproteins importantgrowth widelyprokaryote finger PLaY inbothanimalsand ofzinc isC2H2 development plants.Thebiggestsubfamilyfin...
(ZFs)areproteinstructuresstabilized bythecoordinatedbindingofazincion.Althoughthere are20differenttypesofZFdomains,eachcategorizedby thestructureoftheirzincstabilizingaminoacids[1,2], themostcommontypeistheCys2-His2(C2H2)type (Fig.1).TheC2H2,or‘‘classical’’zincfinger,comprisea largegroupofproteins...