目的探讨miR-155在N-3-氧代十二烷酰-L-同型丝氨酸内酯(3-oxo-C12-HSL)阻碍人单核细胞源性树突状细胞(Mo-DCs)成熟过程中的表达变化.方法 分离正常人外周血CD14+单核细胞,经重组人粒-单核细胞集落刺激因子和重组人IL-4诱导分化为Mo-DCs.将不成熟Mo-DCs分为阴性对照组,模型组,实验组1,实验组2,阴性对照...
3-oxo-C12:2-HSL is structurally close to 3-oxo-C12-HSL synthesized by Pseudomonas aeruginosa, a molecule whose effects on host cells are well reported in the literature15,16. We previously described the anti-inflamma- tory and dose-dependent effects of 3-oxo-C12:2-HSL on intestinal ...
目的 探讨3-oxo-C12-HSL对MH-S小鼠肺泡巨噬细胞自噬的影响.方法 用LasI基因(3-oxo-C12-HSL合成基因)缺失型和野生型的铜绿假单胞菌(PA)菌株的培养物上清液以及化学合成的3-oxo-C12-HSL信号分子处理MH-S细胞,激光共聚焦荧光显微镜观察LC3-GFP荧光斑点以及Western blot检测LC3Ⅱ/LC3Ⅰ比值来监测自噬的形成,同时...
Growth curves and 3-oxo-C12-HSL autoinducer concentrations in culture supernatants.Thilo KöhlerHajer OuertataniSakouhiPierre CossonChristian van Delden
Antagonistic activity of F7 against 3-oxo-C12HSL mediated QS in E. coli bioreporter strain harboring pSC11(PlasI)::lacZ) and lasR expression vector pJN105L.Sajal, SarabhaiPrince, SharmaNeena, Capalash
Furvina inhibits the 3-oxo-C12-HSL-based quorum sensing system of Pseudomonas aeruginosa and QS-dependent phenotypesAntimicrobial resistanceantivirulencebiofilmfurvinaquorum sensing inhibitionDisruption of cell-cell communication or quorum sensing (QS) is considered a stimulating approach...