目的 探讨3-oxo-C12-HSL对MH-S小鼠肺泡巨噬细胞自噬的影响.方法 用LasI基因(3-oxo-C12-HSL合成基因)缺失型和野生型的铜绿假单胞菌(PA)菌株的培养物上清液以及化学合成的3-oxo-C12-HSL信号分子处理MH-S细胞,激光共聚焦荧光显微镜观察LC3-GFP荧光斑点以及Western blot检测LC3Ⅱ/LC3Ⅰ比值来监测自噬的形成,同时...
www.nature.com/scientificreports OPEN 3‑oxo‑C12:2‑HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro‑inflammatory pathways in immune cells via bitter taste receptors Garance Coquant1,2, Doriane Aguanno1,2,3, Loïc Brot1,2, Christine Belloir4, ...
Conclusion 3-O-C12-HSL can bind to PPARγ, and then inhibit the secretion of IL-6 by Mo-DCs through it. Key words: N- 3- oxododecanoyl- L- homoserine lactone; monocytes derived- dendritic cells; peroxisome proliferater- activated receptor γ; interleukin-6 铜绿假单胞菌(Pseudomonas aerlt...
目的 研究铜绿假单胞菌分泌的N-3-氧代十二烷酰-L-同型丝氨酸内酯(N-3-oxododecanoyl-L-homoserine lactone,3-O-C12-HSL)是否通过过氧化物酶增殖物激活受体γ(peroxisome proliferater-activated receptorγ,PPARγ)调节单核细胞来源的树突细胞(monocytes derived-dendritic cells,Mo-DCs)表达白细胞介素6(interleukin...
目的研究铜绿假单胞菌分泌的N-3-氧代十二烷酰-L-同型丝氨酸内酯(N-3-oxododecanoyl-L-homoserine lactone,3-O-C12-HSL)是否通过过氧化物酶增殖物激活受体γ(peroxisome proliferater-activated receptorγ,PPARγ)调节单核细胞来源的树突细胞(monocytes derived-dendritic cells,Mo-DCs)表达白细胞介素6(interleukin-...
目的制备纯化的人过氧化物酶体增殖物激活受体-配体结合域(PPARγ-LBD)肽段并观察铜绿假单胞菌信号分子N-3-氧代十二烷酰-L-同型丝氨酸内酯(3-O-C_(12)-HSL)与PPARγ-LB... 张云燕,马刘合一,陈敏怡,... - 《天津医药》 被引量: 0发表: 2022年 Pseudomonas signal molecule 3-oxo-C12-homoserine lacto...
单核细胞来源的树突状细胞目的探讨miR-155在N-3-氧代十二烷酰-L-同型丝氨酸内酯(3-oxo-C12-HSL)阻碍人单核细胞源性树突状细胞(Mo-DCs)成熟过程中的表达变化.方法 分离正常人外周血CD14+单核细胞,经重组人粒-单核细胞集落刺激因子和重组人IL-4诱导分化为Mo-DCs.将不成熟Mo-DCs分为阴性对照组,模型组,...
In this study, the antibiotic furvina was shown to be able to cause inhibition of the 3-oxo-C12-HSL-dependent QS system of Pseudomonas aeruginosa. Furvina interacts with the LasI/LasR system. The data were validated by modeling studies. Furvina can also reduce biofilm formation and decrease ...
aeruginosa associated 3-oxo-C 12 -HSL exerts the inhibitory effect on S. epidermidis growth and biofilms and thus it may also help Pseudomonas to dominate under the co-infection conditions.doi:10.1016/j.micpath.2017.08.009Singh, Pradeep Kumar...
Growth curves and 3-oxo-C12-HSL autoinducer concentrations in culture supernatants.Thilo KöhlerHajer OuertataniSakouhiPierre CossonChristian van Delden