此外,肿瘤细胞系A549,PC9和H1299具有更高的EMS拷贝数。 2 .EMS是c-Myc的直接转录目标 通过RNA测序分析,EMS被鉴定为c-Myc反应性lncRNA。证明c-Myc的诱导增加,而c-Myc的敲低减少,A549,PC9,H1299,HCT116和MCF7细胞中的EMS表达,表明c-My...
此外,肿瘤细胞系A549,PC9和H1299具有更高的EMS拷贝数。 2 .EMS是c-Myc的直接转录目标 通过RNA测序分析,EMS被鉴定为c-Myc反应性lncRNA。证明c-Myc的诱导增加,而c-Myc的敲低减少,A549,PC9,H1299,HCT116和MCF7细胞中的EMS表达,表明c-Myc对EMS表达的特异性作用。根据c-Myc和EMS细胞表达之间的关系,对TCGA数据...
Immunohistochemical staining was used to detect MYH9 expression in a tissue microarray containing 49 NSCLC and 43 adjacent tissue specimens. MYH9 knockout cell models were established in H1299 and H1975 cells using CRISPR/Cas9 technology, and the changes in cell proliferation cell were assessed using...
b, c, A549 and H1299 cells were cultured in low-glucose medium and were then treated with or without [U-13C2]-acetate or unlabeled acetate. Intracellular acetate (b) and acetyl-CoA (c) were extracted and measured. d, e, A549 and H1299 cells expressing different shRNAs targeting MCT1,...
Wu C, Wang S, Xu C, Tyler A, Li X, Andersson C, Oji Y, Sugiyama H, Chen Y and Li A: WT1 enhances proliferation and impedes apoptosis in KRAS mutant NSCLC via targeting cMyc. Cell Physiol Biochem 35: 647‑662, 2015....
Fadu cells were cultured in a 6-well plate, when reached 100% confluence, used a 200 μL pipette tip to form stripes on the monolayer and cultured in serum-free DMEM medium for 24 h. Images of the cells were captured at 0 and 24 h after form stripes. Transwell migration assay...
The lung cancer cell lines H1299, H460, and A549 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The lung cancer cell line H520, human bronchial epithelial cells (HBE), and human normal lung epithelial cells (BEAS-2B) were routinely conserved in ou...
LINC01123 functions as an oncogene via miR-199a-5p and c-Myc. a Colony formation rescue experiment showed that cell proliferation reduced by si-1123 could be increased by miR-199a-5p inhibitor or Myc-OE in H1299 cell. b Colony formation rescue experiment showed that cell proliferation stimul...
Ghosh等[20]将表达 MBP-1的复制缺陷型重组腺病毒AdMBP-1转染非小细胞肺癌细胞株H1299,发现MBP-1的过表达能显著抑制肺癌细胞的增殖活性,给裸鼠皮下注射H1299细胞复制非小细胞肺癌移植瘤动物模型,当肿瘤体积达184 mm3时, 给荷瘤裸鼠每天注射单剂重组腺病毒AdMBP-1,连续注射五天,观察其治疗作用,结果发现,接受重组...
Glucose deprivation differentially affects c-MYC protein stability in HeLa and MDA-MB-231 cells. (a) Western blot detection of c-MYC in HeLa and MDA-MB-231 cells treated with CHX (0.1 mM) and MG-132 (10 μM) in the presence or absence of 25 mM glucose for 12 h. (b) HEK293T ...