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5605S quantity : 100 ul price : 260 USD clonality : monoclonal host : rabbit reactivity : ,human,mouse,rat,dog,pig,rabbit application : western blot,immunocytochemistry,immunoprecipitation,flow cytometry,immunohistochemistry - paraffin section
#5605 Citations (1030) Filter: WB IF Western blot analysis of extracts from control HEK293 cells (lane 1) or c-Myc knockout HEK293 cells (lane 2) using c-Myc (D84C12) Rabbit mAb Antibody, #5605 (upper) or β-actin (13E5) Rabbit mAb, #4970 (lower). The absence of signal...
Lysates were incubated with monoclonal anti-MYC (5605S, Cell Signaling) overnight at 4°C, then protein-G sepharose beads (Millipore) for another 2 h at 4°C. Immunoprecipitates were washed 4 times with immunoprecipitation lysate buffer and resuspended in 90 μl 2 × SDS loading buffer. ...
使用以下抗体进行 蛋白质印迹分析:i)c-Myc#5605(兔单克隆,1:2000稀释,(Cell Signaling Technologies));肌动蛋白#A2228(小鼠单克隆,1:5000稀释(Sigma))、PP2A#610555(小鼠 单克隆,1:5000稀释,BD Biosciences)、p-PP2A#1155-1(兔多克隆,1:5000稀释, (Epitomics))、Erk#9107(小鼠单克隆,1:2000稀释(Cell ...
(批号MM-71308R2)购自江苏酶免实业有限公司;乳酸试剂盒(批号FY40175-B)购自江苏菲亚生物科技有限公司;SIRT1抗体(批号2028)、Myc原癌基因(cancer-Myc,c-Myc)抗体(批号5605)、乳酸脱氢酶A(lactate dehydrogenase A,LDHA)抗体(批号2012...
and stained with α-tubulin (Cell Signalling 3873, 1:3000 and Cell Signalling 4413 1:1000) and cMyc (Cell Signalling 5605, 1:1000 and Cell Signalling 4408 1:1000) antibodies followed by DAPI Prolong gold antifade (Invitrogen P36931). All confocal images were acquired using Zeiss LSM 880 ...
(1:1,000, 5605) from Cell Signaling Technology (Beverly, MA, USA); Vimentin (1:3,000, sc-32322), Snail (1:1,000, sc-28199), ZEB1 (1:2,000, sc-25388), ZEB2 (1:1,000, sc-48789), CBP (1:2,000, sc-369), DNMT1 (1:1,000, sc-20701), HDAC1 (1:2,000, sc-7872), ...
FBP1 antibody (ab109732)was purchased from Abcam (working dilution 1:1000); beta-Tubulin (2128S) was from Cell Signaling Technology - (working dilution 1:5000); TRIM28 (ab10483) was from Abcam (working dilution 1:3000); c-Myc (5605P) was from Santa Cruz Biotechnology (working dilution ...
5595-5605 View in ScopusGoogle Scholar [39] J. Zhang, L. Kong, S. Guo, M. Bu, Q. Guo, Y. Xiong, N. Zhu, C. Qiu, X. Yan, Q. Chen, H. Zhang, J. Zhuang, Q. Wang, S.S. Zhang, Y. Shen, M. Chen hnRNPs and ELAVL1 cooperate with uORFs to inhibit protein translation ...